Evidence map›Paper›PMID 33726664›Full record

ArticleBMC genomics2021

Development and validation of genome-wide InDel markers with high levels of polymorphism in bitter gourd (Momordica charantia).

Junjie Cui, Jiazhu Peng, Jiaowen Cheng, Kailin Hu

Abstract read
In one paragraph

Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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3citing papers in PubMed
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3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Genome-wide identification and development of InDel markers in tobacco (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022
    Article
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5 · Who and what money

Authors and funding

4 authors.

Junjie Cui *Department of Horticulture, Foshan University, Foshan, Guangdong, 528225, People's Republic of China.
Jiazhu Peng *Guangzhou Academy of Agricultural Sciences, Guangzhou, Guangdong, 510335, People's Republic of China.
Jiaowen ChengCollege of Horticulture, South China Agricultural University, Guangzhou, Guangdong, 510642, People's Republic of China.
Kailin HuCollege of Horticulture, South China Agricultural University, Guangzhou, Guangdong, 510642, People's Republic of China. hukailin@scau.edu.cn.

Funding

Guangdong Basic and Applied Basic Research Foundation 2019A1515011939Key Project of Basic and Applied Research for Ordinary Universities of Guangdong Province 2018KZDXM016Science and Technology Planning Project of Guangdong Province 2018B020202007Scientific Research Foundation for Talented Scholars of Foshan University CGG07127
6 · The paper itself

Abstract

backgroundThe preferred choice for molecular marker development is identifying existing variation in populations through DNA sequencing. With the genome resources currently available for bitter gourd (Momordica charantia), it is now possible to detect genome-wide insertion-deletion (InDel) polymorphisms among bitter gourd populations, which guides the efficient development of InDel markers.

resultsHere, using bioinformatics technology, we detected 389,487 InDels from 61 Chinese bitter gourd accessions with an average density of approximately 1298 InDels/Mb. Then we developed a total of 2502 unique InDel primer pairs with a polymorphism information content (PIC) ≥0.6 distributed across the whole genome. Amplification of InDels in two bitter gourd lines '47-2-1-1-3' and '04-17,' indicated that the InDel markers were reliable and accurate. To highlight their utilization, the InDel markers were employed to construct a genetic map using 113 '47-2-1-1-3' × '04-17' F

conclusionsThis is the first report on the development of genome-wide InDel markers for bitter gourd. The validation of the amplification and genetic map construction suggests that these unique InDel markers may enhance the efficiency of genetic studies and marker-assisted selection for bitter gourd.

Indexed as

Momordica charantiaGenetic LinkageGenomeHumansINDEL MutationSequence Analysis, DNABitter gourdGenetic mapInsertion and deletion (InDel)Molecular markerPolymorphism

Identifiers

PMID33726664
PMCPMC7968231

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