Evidence map›Paper›PMID 33725277›Full record

ArticleGenes & genomics2021

MALAT1 knockdown promoted cell viability and migration of LPS-treated MG-63 cells via sponging miR-212.

Yong Zhang, Yuan Zhang, Kun Yang, Weizhong Guo, Xiaojun Ma, Xingming Ma, Zengliang Wang

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Article in Genes & genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yong Zhang *Department of Trauma and Joint, The Second Hospital of Qinhuangdao, Qinhuangdao, Hebei, 066600, China. zhangyong_tijh@126.com.
Yuan Zhang *Department of Gastroenterology and Nephrology, The Second Hospital of Qinhuangdao, Qinhuangdao, Hebei, 066600, China.
Kun YangDepartment of Trauma and Joint, The Second Hospital of Qinhuangdao, Qinhuangdao, Hebei, 066600, China.
Weizhong GuoDepartment of Trauma and Joint, The Second Hospital of Qinhuangdao, Qinhuangdao, Hebei, 066600, China.
Xiaojun MaDepartment of Trauma and Joint, The Second Hospital of Qinhuangdao, Qinhuangdao, Hebei, 066600, China.
Xingming MaDepartment of Trauma and Joint, The Second Hospital of Qinhuangdao, Qinhuangdao, Hebei, 066600, China.
Zengliang WangDepartment of Osteology, Tianjin Hospital, No. 406, Jiefang South Road, Tianjin, 300211, China. wangzengliang_tijh@163.com.
Qinhuangdao Second Hospital · CNTianjin Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMost fractures could heal after treatment, around 5-10 % of patients still develop delayed union and nonunion. Evidence has increasingly shown that abnormal expression of long noncoding RNAs is closely related to the occurrence and development of various diseases including fracture healing. However, evidence regarding the effect of MALAT1 on fracture healing remains limited.

objectivesIn this study, we attempt to explore the role of MALAT1 during the process of femoral neck fracture healing and elucidate the underlying mechanism of this disease.

methodsWe first detect the expression of lncRNAs in serums from 3 pairs of patients with delayed femoral neck fracture healing and healthy volunteers using lncRNA microarray. And the expression of long noncoding RNA MALAT1 in serums and LPS-treated MG-63 cells was measured using qRT-PCR. CCK-8 assay, cell migration and qRT-PCR were applied to the role of MALAT1 knockdown in LPS-treated MG-63 cells. ELISA was used for the measurement of inflammatory cytokines in serums of patients and healthy volunteers. The bioinformatics analysis and the rescue experiment were devoted to the underlying mechanism.

resultsMALAT1 expression was up-regulated in serum of patients with delayed union of femoral neck fracture. MALAT1 knockdown promoted cell viability and migration, reduced inflammation in LPS-treated MG-63 cells. The bioinformatics analysis showed MALAT1 acts as a molecular sponge for miR-212. And SOX6 was a target of miR-212. Besides, MALAT1 knockdown suppressed SOX6 expression via targeting miR-212 in LPS-treated MG-63 cells.

conclusionsThese data suggest MALAT1 knockdown promoted the biological behavior of LPS-treated MG-63 cells via sponging miR-212, which may provide a new therapeutic avenue for delayed union of femoral neck fracture.

Indexed as

Cell MovementAdolescentAdultCell Line, TumorCell SurvivalFemaleFemoral Neck FracturesHumansLipopolysaccharidesMaleMicroRNAsMiddle AgedOsteoblastsRNA, Long NoncodingSOXD Transcription FactorsUp-RegulationLipopolysaccharidesMALAT1 long non-coding RNA, humanMicroRNAsMIRN212 microRNA, humanRNA, Long NoncodingSOX6 protein, humanSOXD Transcription FactorsFemoral neck fractureMALAT1miR-212SOX6

Identifiers

PMID33725277
OpenAlexW3137217462

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.