ArticleHistochemistry and cell biology2021
Relationship between serum concentration, functional parameters and cell bioenergetics in IPEC-J2 cell line.
Article in Histochemistry and cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Culture Optimization of the IPEC-J2 Piglet Jejunum Cell Line for Applications in Infant Nutrition Research.Molecular nutrition & food research · 2026Article
- IPEC-J2 as a cellular model for studying intestinal mucus.Scientific reports · 2025Article
- Lactate alleviates intestinal barrier injury in weaned piglets via activation of the Wnt/β-catenin pathway and promotion of intestinal epithelial cell proliferation.Journal of animal science and biotechnology · 2025Article
- IMDM-20 enhances neutrophilic features during 1.3 % DMSO-mediated differentiation of HL-60 cells.Biochemistry and biophysics reports · 2025Article
- Improving porcine in vitro blastocyst development using fetal bovine serum, amino acids, and insulin-transferrin-selenium.The Journal of reproduction and development · 2025Article
- Immunomodulatory effect of probiotic exopolysaccharides in a porcine in vitro co-culture model mimicking the intestinal environment on ETEC infection.Veterinary research communications · 2024Article
- Fetal bovine serum contains biologically available ATP.Purinergic signalling · 2024Article
- Saturated fatty acids differently affect mitochondrial function and the intestinal epithelial barrier depending on their chain length in theFrontiers in cell and developmental biology · 2024Article
- Employing active learning in the optimization of culture medium for mammalian cells.NPJ systems biology and applications · 2023Article
- Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm.Reproduction in domestic animals = Zuchthygiene · 2023Article
- The Impairment of Cell Metabolism by Cardiovascular Toxicity of Doxorubicin Is Reversed by Bergamot Polyphenolic Fraction Treatment in Endothelial Cells.International journal of molecular sciences · 2022Article
- Mitochondria Bioenergetic Functions and Cell Metabolism Are Modulated by the Bergamot Polyphenolic Fraction.Cells · 2022Article
- Development of a Pig Mammary Epithelial Cell Culture Model as a Non-Clinical Tool for Studying Epithelial Barrier-A Contribution from the IMI-ConcePTION Project.Animals : an open access journal from MDPI · 2021Article
- Vitamin K Vitamers Differently Affect Energy Metabolism in IPEC-J2 Cells.Frontiers in molecular biosciences · 2021Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The foetal bovine serum (FBS) concentration could influence functional parameters of IPEC-J2 cells. IPEC-J2 is a non-transformed continuous epithelial cell line that represents an established in vitro model to study porcine gut inflammation and alterations of intestinal integrity. This cell line also represents a good translational model thanks to the high similitudes between pig and human gastrointestinal tract. With the aim to assess if the FBS-dependent functional variations are linked to the bioenergetic aspects, the addition of 5% and 10% FBS in the IPEC-J2 culture medium were tested. Doubling time and TEER measurement indicated that cells cultured at higher FBS dose grow faster and as a more compact monolayer. 10% FBS increases ATP production and mitochondrial oxidative phosphorylation (OxPhos) and does not affect glycolysis. Both at 5% and 10% FBS ATP production mainly comes from OxPhos and FBS concentration does not affect the cell respiration bioenergetic parameters. Noteworthy, IPEC-J2 treated with 5% and 10% FBS have a metabolic potential since both OxPhos and glycolysis increase by > 100% and < 50%, respectively in comparison with baseline metabolism. Moreover, glucose, fatty acids and glutamine constitute the preferred metabolic fuel for mitochondrial respiration at both FBS conditions tested. Accordingly, the cells flexibility to oxidize these substrates shows that IPEC-J2 mitochondria cannot maintain the basal ATP production without oxidizing all the substrates available irrespective of FBS concentration. To sum up, in IPEC-J2 cells OxPhos increases with the FBS-stimulated functional physiological parameters to fulfil ATP requirements.
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