ArticleDevelopment (Cambridge, England)2021
Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo.
Article in Development (Cambridge, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Dynamics of bicoid mRNA localisation and translation dictate morphogen gradient formation.The EMBO journal · 2026Article
- Real-time single-molecule imaging in zebrafish embryos uncovers noncanonical translation.Science advances · 2026Article
- SunTag-Based Single-Molecule Translation Imaging inBio-protocol · 2025Article
- Measuring and manipulating localized translation of erm-1 in the C. elegans embryo.Development (Cambridge, England) · 2025Article
- The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.Nature reviews. Genetics · 2025Review
- Expanding the tagging toolbox for visualizing translation live.The Biochemical journal · 2025Review
- Imaging Translation in Early Embryo Development.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A simple MiMIC-based approach for tagging endogenous genes to visualise live transcription in Drosophila.Development (Cambridge, England) · 2024Article
- Article
- Boosting the toolbox for live imaging of translation.RNA (New York, N.Y.) · 2024Article
- Direct observation of translational activation by a ribonucleoprotein granule.Nature cell biology · 2024Article
- Translation Dynamics of Single mRNAs in Live Cells.Annual review of biophysics · 2024Review
- Review
- Vasa, a regulator of localized mRNA translation on the spindle.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Review
- Combined modelling of mRNA decay dynamics and single-molecule imaging in the Drosophila embryo uncovers a role for P-bodies in 5' to 3' degradation.PLoS biology · 2023Article
- Modelling the structure of Short Gastrulation and generation of a toolkit for studying its function in Drosophila.Biology open · 2022Article
- Studying Protein Function Using Nanobodies and Other Protein Binders in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Application of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression inLife (Basel, Switzerland) · 2021Article
- Live and fixed imaging of translation sites at single mRNA resolution in theSTAR protocols · 2021Article
- Modulating the bicoid gradient in space and time.Hereditas · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The Hunchback (Hb) transcription factor is crucial for anterior-posterior patterning of the Drosophila embryo. The maternal hb mRNA acts as a paradigm for translational regulation due to its repression in the posterior of the embryo. However, little is known about the translatability of zygotically transcribed hb mRNAs. Here, we adapt the SunTag system, developed for imaging translation at single-mRNA resolution in tissue culture cells, to the Drosophila embryo to study the translation dynamics of zygotic hb mRNAs. Using single-molecule imaging in fixed and live embryos, we provide evidence for translational repression of zygotic SunTag-hb mRNAs. Whereas the proportion of SunTag-hb mRNAs translated is initially uniform, translation declines from the anterior over time until it becomes restricted to a posterior band in the expression domain. We discuss how regulated hb mRNA translation may help establish the sharp Hb expression boundary, which is a model for precision and noise during developmental patterning. Overall, our data show how use of the SunTag method on fixed and live embryos is a powerful combination for elucidating spatiotemporal regulation of mRNA translation in Drosophila.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.