ArticlePlant physiology2021
Estimating the number of protein molecules in a plant cell: protein and amino acid homeostasis during drought.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 82 citations in OpenAlex.
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- The Effects of Rapid Mitochondrial Gene Loss on Organellar Proteomes.Genome biology and evolution · 2026Article
- Quantitative Proteomic Profiling of Pinctada fucata Shell Nacre Defines a Solubility-Based Type Classification of Shell Matrix Proteins.Marine biotechnology (New York, N.Y.) · 2026Article
- Reshaping organellar translation and tRNA metabolism: the consequences of photosynthesis loss and massive horizontal gene transfer.Molecular biology and evolution · 2026Article
- Single-cell proteomics of Arabidopsis leaf mesophyll reveals dynamic protein responses to water-deficit stress.Genome biology · 2026Article
- Capturing protein-protein interactions in plants: recent advances, challenges, and opportunities.Frontiers in molecular biosciences · 2026Review
- Mechanistic insights into the delayed yellowing of broccoli by the combination of ectoine and lactide peptide.NPJ science of food · 2025Article
- Adapting Crops to Rising Temperatures: Understanding Heat Stress and Plant Resilience Mechanisms.International journal of molecular sciences · 2025Review
- Plant MutS2 proteins function in plastid ribosome quality control.bioRxiv : the preprint server for biology · 2025Article
- A Ketol-Acid Reductoisomerase Inhibitor That Has Antituberculosis and Herbicidal Activity.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Mechanisms of Salt and Drought Stress Responses in Foxtail Millet.Plants (Basel, Switzerland) · 2025Review
- Abundant clock proteins point to missing molecular regulation in the plant circadian clock.Molecular systems biology · 2025Article
- The Involvement of Amino Acid Metabolism in the Mechanisms of Salt Tolerance Adaptation inPlants (Basel, Switzerland) · 2025Article
- Drought Adaptation and Responses ofEcology and evolution · 2025Article
- Purification of SlREC2 from wild-type tomato leaflets using immunoaffinity chromatography and immunoprecipitation.Frontiers in plant science · 2025Article
- Applications of the wheat germ cell-free protein synthesis system in plant biochemical studies.Plant biotechnology (Tokyo, Japan) · 2024Article
- Integrating Morpho-Physiological, Biochemical, and Molecular Genotyping for Selection of Drought-Tolerant Pigeon Pea (Plants (Basel, Switzerland) · 2024Article
- Deep proteomics reveals incorporation of unedited proteins into mitochondrial protein complexes in Arabidopsis.Plant physiology · 2024Article
- Copper Oxide Nanoparticles Induced Growth and Physio-Biochemical Changes in Maize (Plants (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During drought stress, cellular proteostasis on the one hand and amino acid homeostasis on the other hand are severely challenged, because the decrease in photosynthesis induces massive proteolysis, leading to drastic changes in both the proteome and the free amino acid pool. Thus, we selected progressive drought stress in Arabidopsis (Arabidopsis thaliana) as a model to investigate on a quantitative level the balance between protein and free amino acid homeostasis. We analyzed the mass composition of the leaf proteome based on proteomics datasets, and estimated how many protein molecules are present in a plant cell and its subcellular compartments. In addition, we calculated stress-induced changes in the distribution of individual amino acids between the free and protein-bound pools. Under control conditions, an average Arabidopsis mesophyll cell contains about 25 billion protein molecules, of which 80% are localized in chloroplasts. Severe water deficiency leads to degradation of more than 40% of the leaf protein mass, and thus causes a drastic shift in distribution toward the free amino acid pool. Stress-induced proteolysis of just half of the 340 million RubisCO hexadecamers present in the chloroplasts of a single mesophyll cell doubles the cellular content of free amino acids. A major fraction of the amino acids released from proteins is channeled into synthesis of proline, which is a compatible osmolyte. Complete oxidation of the remaining fraction as an alternative respiratory substrate can fully compensate for the lack of photosynthesis-derived carbohydrates for several hours.
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