ArticlePlant physiology2021
Host sunflower-induced silencing of parasitism-related genes confers resistance to invading Orobanche cumana.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 30 citations in OpenAlex.
- Large-scale exchange of RNA between parasitic plant Orobanche cumana and its host Helianthus annuus.Pest management science · 2026Article
- Functional Analysis of the GH16 Domain-ContainingPlants (Basel, Switzerland) · 2026Article
- Parasitic Plant-Host Interactions: Molecular Mechanisms and Agricultural Resistance Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Agrobacterium-mediated transformation for virus-induced gene silencing (VIGS) in Castilleja tenuiflora: Cte-chlH and Cte-PDS.Plant cell reports · 2025Article
- Parasitic plant Cistanche tubulosa shapes the bacterial community structure and functional composition of the salt-tolerant host Tamarix chinensis across different parasitic statuses.Environmental microbiome · 2025Article
- Monitoring of parasite Orobanche cumana using Vis-NIR hyperspectral imaging combining with physio-biochemical parameters on host crop Helianthus annuus.Plant cell reports · 2024Article
- Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol.Plant methods · 2024Article
- Integrating cotyledon-based virus-induced gene silencing with visual marker promises a rapid, highly effective validation of gene functions inFrontiers in plant science · 2024Article
- Physiology and transcriptomics highlight the underlying mechanism of sunflower responses to drought stress and rehydration.iScience · 2023Article
- From guest to host: parasite Cistanche deserticola shapes and dominates bacterial and fungal community structure and network complexity.Environmental microbiome · 2023Article
- Large-scale mRNA transfer betweeniScience · 2023Article
- Analysis of public domain plant transcriptomes expands the phylogenetic diversity of the family Secoviridae.Virus genes · 2022Article
- Host-Induced Gene Silencing of a G Protein α Subunit GeneJournal of fungi (Basel, Switzerland) · 2021Article
- Effects of Benzoquinones on Radicles ofPlants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Orobanche cumana is a holoparasitic plant that attaches to host-plant roots and seriously reduces the yield of sunflower (Helianthus annuus L.). Effective control methods are lacking with only a few known sources of genetic resistance. In this study, a seed-soak agroinoculation (SSA) method was established, and recombinant tobacco rattle virus vectors were constructed to express RNA interference (RNAi) inducers to cause virus-induced gene silencing (VIGS) in sunflower. A host target gene HaTubulin was systemically silenced in both leaf and root tissues by the SSA-VIGS approach. Trans-species silencing of O. cumana genes were confirmed for 10 out of 11 target genes with silencing efficiency of 23.43%-92.67%. Knockdown of target OcQR1, OcCKX5, and OcWRI1 genes reduced the haustoria number, and silencing of OcEXPA6 caused further phenotypic abnormalities such as shorter tubercles and necrosis. Overexpression of OcEXPA6 caused retarded root growth in alfalfa (Medicago sativa). The results demonstrate that these genes play an important role in the processes of O. cumana parasitism. High-throughput small RNA (sRNA) sequencing and bioinformatics analyses unveiled the distinct features of target gene-derived siRNAs in O. cumana such as siRNA transitivity, strand polarity, hotspot region, and 21/22-nt siRNA predominance, the latter of which was confirmed by Northern blot experiments. The possible RNAi mechanism is also discussed by analyzing RNAi machinery genes in O. cumana. Taken together, we established an efficient host-induced gene silencing technology for both functional genetics studies and potential control of O. cumana. The ease and effectiveness of this strategy could potentially be useful for other species provided they are amenable to SSA.
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