ArticleNucleic acids research2021
A ProQ/FinO family protein involved in plasmid copy number control favours fitness of bacteria carrying mcr-1-bearing IncI2 plasmids.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Genome-wide characterization of host factors involved in single-stranded RNA and DNA phage infection pathways.bioRxiv : the preprint server for biology · 2026Article
- A novel ABC family protein participates in the regulation of fitness cost caused byFundamental research · 2026Article
- Tracking Global Transmission Dynamics of the Plasmid-MediatedMicroorganisms · 2025Article
- A lignan compound regulates LPS modifications via PmrA/B signaling cascades to potentiate colistin efficacy in vivo.PLoS pathogens · 2025Article
- Genomic evolution and stability of the mcr-1-harboring IncI2 plasmid in the presence and absence of colistin.BMC genomics · 2025Article
- Flavomycin inhibits plasmid-mediated conjugative transfer of antibiotic resistance genes by disrupting energy metabolism and pilus assembly.Zoological research · 2025Article
- Colistin resistance plasmids dually enhance bacterial virulence and antibiotic resistance via surface polysaccharide biosynthesis.Nature communications · 2025Article
- DNA Polymerase IV dinB Favors the Adaptive Fitness of mcr-carrying Bacteria Through a Negative Feedback Regulatory Mechanism.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Genomic diversity of mcr-carrying plasmids and the role of type IV secretion systems in IncI2 plasmids conjugation.Communications biology · 2025Article
- VirBR counter-silences HppX3 to promote conjugation of blaNDM-IncX3 plasmids.Nucleic acids research · 2025Article
- A noncanonical intrinsic terminator in the HicAB toxin-antitoxin operon promotes the transmission of conjugative antibiotic resistance plasmids.Nucleic acids research · 2025Article
- The global prevalence, formation, and evolutionary inference of bacteria co-carrying theFrontiers in microbiology · 2025Article
- A plasmid with theMicrobiology (Reading, England) · 2025Article
- The multifaceted roles of phosphoethanolamine-modified lipopolysaccharides: from stress response and virulence to cationic antimicrobial resistance.Microbiology and molecular biology reviews : MMBR · 2024Review
- Porin deficiency or plasmid copy number increase mediated carbapenem-resistantEmerging microbes & infections · 2024Article
- Article
- Compensatory evolution of chromosomes and plasmids counteracts the plasmid fitness cost.Ecology and evolution · 2024Article
- VirBR, a transcription regulator, promotes IncX3 plasmid transmission, and persistence of blaNature communications · 2024Article
- Flavomycin restores colistin susceptibility in multidrug-resistant Gram-negative bacteria.mSystems · 2024Article
- Coexistence of a nonresistance-conferring IncI1 plasmid favors persistence of theMicrobiology spectrum · 2024Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The plasmid-encoded colistin resistance gene mcr-1 challenges the use of polymyxins and poses a threat to public health. Although IncI2-type plasmids are the most common vector for spreading the mcr-1 gene, the mechanisms by which these plasmids adapt to host bacteria and maintain resistance genes remain unclear. Herein, we investigated the regulatory mechanism for controlling the fitness cost of an IncI2 plasmid carrying mcr-1. A putative ProQ/FinO family protein encoded by the IncI2 plasmid, designated as PcnR (plasmid copy number repressor), balances the mcr-1 expression and bacteria fitness by repressing the plasmid copy number. It binds to the first stem-loop structure of the repR mRNA to repress RepA expression, which differs from any other previously reported plasmid replication control mechanism. Plasmid invasion experiments revealed that pcnR is essential for the persistence of the mcr-1-bearing IncI2 plasmid in the bacterial populations. Additionally, single-copy mcr-1 gene still exerted a fitness cost to host bacteria, and negatively affected the persistence of the IncI2 plasmid in competitive co-cultures. These findings demonstrate that maintaining mcr-1 plasmid at a single copy is essential for its persistence, and explain the significantly reduced prevalence of mcr-1 following the ban of colistin as a growth promoter in China.
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