ArticlePloS one2021
Rapid detection of extended spectrum β-lactamase producing Escherichia coli isolated from fresh pork meat and pig cecum samples using multiplex recombinase polymerase amplification and lateral flow strip analysis.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Antimicrobial resistance in meat and meat products from Asia: An urgent one health challenge- a systematic review and meta-analysis.Poultry science · 2025Pooled it
- Duplex Recombinase Polymerase Amplification-Lateral Flow Assay for Rapid Visual Detection of Critical Antibiotic Resistance Genes in Escherichia coli and Klebsiella pneumoniae From Positive Blood Cultures.Journal of clinical laboratory analysis · 2026Article
- Commensal and opportunistic bacteria resistant to fourth-generation cephalosporins or fluoroquinolones isolated from yellow-legged gulls (Larus michahellis) settled in Taranto, Southern Italy.Scientific reports · 2026Article
- Tracking ESBL-ProducingVeterinary sciences · 2026Article
- Article
- Article
- Polymerase Chain Reaction-Lateral Flow Strip for DetectingAntibiotics (Basel, Switzerland) · 2025Article
- Development of a Protein-Free Nucleic Acid Lateral Flow Assay forAnalytical chemistry · 2025Article
- Development of a PCR-dipstick DNA chromatography-based tool for the detection of CTX-M- and TEM-producing Escherichia coli and Klebsiella pneumoniae isolated from patients in Kafue and Katete districts of Zambia.BMC infectious diseases · 2025Article
- Validation of Recombinase Polymerase Amplification with In-House Lateral Flow Assay forAntibiotics (Basel, Switzerland) · 2024Article
- Phenotypic and Genotypic Characterization ofAntibiotics (Basel, Switzerland) · 2024Article
- Antimicrobial Susceptibility of CommensalAnimals : an open access journal from MDPI · 2024Article
- The Global Rise of ESBL-ProducingAnimals : an open access journal from MDPI · 2024Review
- A Health Threat from Farm to Fork: Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2024Article
- Identification and Genomic Analyses of a Multidrug Resistant Avian PathogenicTransboundary and emerging diseases · 2024Article
- Antimicrobial Resistance in Bacteria from Meat and Meat Products: A One Health Perspective.Microorganisms · 2023Review
- Detection of Extended-spectrum β-lactamase-producing Escherichia coli isolates by isothermal amplification and association of their virulence genes and phylogroups with extraintestinal infection.Scientific reports · 2023Article
- Co-Occurrence ofAntibiotics (Basel, Switzerland) · 2023Article
- Improvement of Seed-Mediated Growth of Gold Nanoparticle Labels for DNA Membrane-Based Assays.Biosensors · 2022Article
- Evaluation of Retail Meat as a Source of ESBLAntibiotics (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emergence and dissemination of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli is a global health issue. Food-producing animals, including pigs, are significant reservoirs of antimicrobial resistance (AMR), which can be transmitted to humans. Thus, the rapid detection of ESBLs is required for efficient epidemiological control and treatment. In this study, multiplex recombinase polymerase amplification (RPA) combined with a single-stranded tag hybridization chromatographic printed-array strip (STH-PAS), as a lateral flow strip assay (LFA), was established for the rapid and simultaneous detection of multiple bla genes in a single reaction. Visible blue lines, indicating the presence of the blaCTX-M, blaSHV, and blaOXA genes, were observed within 10 min by the naked eye. The limit of detection of all three genes was 2.5 ng/25 μL, and no cross-reactivity with seven commensal aerobic bacteria was observed. A total of 93.9% (92/98) and 96% (48/50) of the E. coli isolates from pork meat and fecal samples, respectively, expressed an ESBL-producing phenotype. Nucleotide sequencing of the PCR amplicons showed that blaCTX-M was the most prevalent type (91.3-95.83%), of which the main form was blaCTX-M-55. The sensitivity and specificity of the RPA-LFA were 99.2% and 100%, respectively, and were in almost perfect agreement (κ = 0.949-1.000) with the results from PCR sequencing. Thus, the RPA-LFA is a promising tool for rapid and equipment-free ESBL detection and may facilitate clinical diagnosis in human and veterinary medicine, as well as AMR monitoring and surveillance.
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