ArticlePLoS neglected tropical diseases2021
Opportunistic feeding behaviour and Leishmania infantum detection in Phlebotomus perniciosus females collected in the human leishmaniasis focus of Madrid, Spain (2012-2018).
Article in PLoS neglected tropical diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 33 citations in OpenAlex.
- LeishVet update and current recommendations on feline leishmaniosis.Parasites & vectors · 2026Review
- Retrospective entomological and eco-epidemiological survey of a leishmaniasis outbreak in southern Spain.One health (Amsterdam, Netherlands) · 2026Article
- Integrating molecular tools into leishmaniosis surveillance: evaluation of a commercial qPCR kit in sand fly vectors.Parasites & vectors · 2026Article
- Revisiting the largest human leishmaniasis outbreak in Western Europe (Madrid, Spain): a follow-up entomological study in 2023 and 2024.Parasites & vectors · 2026Article
- Agreement Document for the Study of Leishmaniasis from a One Health Approach in Spain.Tropical medicine and infectious disease · 2025Article
- First clinical cases of leishmaniosis in meerkats (Suricata suricatta) housed in wildlife parks in Madrid, Spain.Parasites & vectors · 2025Article
- Analysis of the Current Risk ofTransboundary and emerging diseases · 2025Article
- Leishmania infantum infection in European badgers (Meles meles) from northeastern Spain: a histopathological and immunohistochemical investigation.Parasitology research · 2024Article
- Spatial Relationship between a Regional Outbreak and a Higher-than-Expected Incidence of Leishmaniasis in Urban Southern Madrid, Spain.The American journal of tropical medicine and hygiene · 2024Article
- A climatic suitability indicator to supportThe Lancet regional health. Europe · 2024Article
- Smelly interactions: host-borne volatile organic compounds triggering behavioural responses in mosquitoes, sand flies, and ticks.Parasites & vectors · 2024Review
- Article
- Infection ofTropical medicine and infectious disease · 2024Article
- Typing ofParasitology · 2024Article
- Multihost/Multivector Community Network: Disentangling Sandfly Species and Host Interactions in Avian Habitats.Transboundary and emerging diseases · 2024Article
- Analysis of the current risk ofFrontiers in veterinary science · 2024Article
- Immunological and genomic characterization of Ibizan Hound dogs in an endemic Leishmania infantum region.Parasites & vectors · 2022Article
- Leishmaniasis vectors in the environment of treated leishmaniasis cases in Spain.Transboundary and emerging diseases · 2022Article
- Stable Episomal TransfectantPathogens (Basel, Switzerland) · 2022Article
- Molecular detection of Leishmania infantum in rats and sand flies in the urban sewers of Barcelona, Spain.Parasites & vectors · 2022Article
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8 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundAn outbreak of human leishmaniasis due to Leishmania infantum has been registered in an urban area of southwestern Madrid, Spain, since 2010. Entomological surveys carried out in the municipalities of Fuenlabrada, Leganés, Getafe and Humanes de Madrid showed that Phlebotomus perniciosus is the only potential vector. In this work, an intensive molecular surveillance was performed in P. perniciosus females captured in the region between 2012 and 2018. METHODOLOGY/PRINCIPAL
findingsA total of 1805 P. perniciosus females were analyzed for Leishmania infection, and 1189 of them also for bloodmeal identification. Eleven different species of vertebrate were detected by amplification and subsequent sequencing of the 359 bp cytb fragment. The most prevalent blood source identified was hare (n = 553, 46.51%), followed by rabbit (n = 262, 21.95%). Less frequent were cat (n = 45, 3.80%), human (n = 34, 2.90%), pig (n = 14, 1.20%), horse (n = 11, 0.93%), sheep (n = 3, 0.25%), rhea (n = 3, 0.25%), partridge (n = 1, 0.09%) and chicken (n = 1, 0.09%). The distribution of the blood meal sources varied between the different locations. Regarding L. infantum detection, PCR amplification of a fragment of kDNA, cpb gene and ITS1 region showed 162 positive specimens (8.97%). The highest infection rate was found in the municipality of Leganés (15.17%).
conclusionsThe results of this molecular survey in P. perniciosus, the only leishmaniasis vector in the outbreak occurred in southwestern Madrid region, showed its opportunistic blood-feeding behaviour, high infection rates and the differences between the different points. This study was an essential part of the intensive surveillance plan in the area and the results obtained have supported the implementation of control measures in the outbreak.
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