ArticleACS sensors2021
Quantification of Single-Molecule Protein Binding Kinetics in Complex Media with Prism-Coupled Plasmonic Scattering Imaging.
Article in ACS sensors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Article
- Label-Free Single-Molecule Immunoassay.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Enhanced plasmonic scattering imaging via deep learning-based super-resolution reconstruction for exosome imaging.Analytical and bioanalytical chemistry · 2024Article
- Label-Free Optical Imaging of Nanoscale Single Entities.ACS sensors · 2024Review
- Label-Free Tracking of Proteins through Plasmon-Enhanced Interference.ACS nanoscience Au · 2024Article
- Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial Resolution.Biosensors · 2024Review
- Plasmonic Scattering Microscopy for Label-Free Imaging of Molecular Binding Kinetics: From Single Molecules to Single Cells.Chemistry methods : new approaches to solving problems in chemistry · 2023Article
- Single-Molecule Optical Biosensing: Recent Advances and Future Challenges.ACS physical chemistry Au · 2023Review
- Multiplexed Protein Detection and Parallel Binding Kinetics Analysis with Label-Free Digital Single-Molecule Counting.Analytical chemistry · 2023Article
- Label-free imaging and biomarker analysis of exosomes with plasmonic scattering microscopy.Chemical science · 2022Article
- Rapid Regulation of Local Temperature and Transient Receptor Potential Vanilloid 1 Ion Channels with Wide-Field Plasmonic Thermal Microscopy.Analytical chemistry · 2022Article
- Single Protein Detection and Imaging with Evanescent Scattering Microscopy.Bio-protocol · 2022Article
- In Situ Analysis of Membrane-Protein Binding Kinetics and Cell-Surface Adhesion Using Plasmonic Scattering Microscopy.Angewandte Chemie (International ed. in English) · 2022Article
- Label-Free Single-Molecule Pulldown for the Detection of Released Cellular Protein Complexes.ACS central science · 2022Article
- Label-free optical biosensors in the pandemic era.Nanophotonics (Berlin, Germany) · 2022Review
- Label-Free Imaging of Single Proteins and Binding Kinetics Using Total Internal Reflection-Based Evanescent Scattering Microscopy.Analytical chemistry · 2022Article
- Evanescent scattering imaging of single protein binding kinetics and DNA conformation changes.Nature communications · 2022Article
- Label-Free Imaging of Nanoscale Displacements and Free-Energy Profiles of Focal Adhesions with Plasmonic Scattering Microscopy.ACS sensors · 2021Article
- Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins.Chemical reviews · 2021Review
- Real-Time analysis of exosome secretion of single cells with single molecule imaging.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Measuring molecular binding is critical for understanding molecular-scale biological processes and screening drugs. Label-free detection technologies, such as surface plasmon resonance (SPR), have been developed for analyzing analytes in their natural forms. However, the specificity of these methods is solely relying on surface chemistry and has often nonspecific binding issues when working with samples in complex media. Herein, we show that single-molecule-based measurement can distinct specific and nonspecific binding processes by quantifying the mass and binding dynamics of individual-bound analyte molecules, thus allowing the binding kinetic analysis in complex media such as serum. In addition, this single-molecule imaging is realized in a commonly used Kretschmann prism-coupled SPR system, thus providing a convenient solution to realize high-resolution imaging on widely used prism-coupled SPR systems.
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Registered trials
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