ArticleChemphyschem : a European journal of chemical physics and physical chemistry2021
Quantitative Assessment of Labeling Probes for Super-Resolution Microscopy Using Designer DNA Nanostructures.
Article in Chemphyschem : a European journal of chemical physics and physical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Nanorulers for Super-Resolution Microscopy.Chembiochem : a European journal of chemical biology · 2026Review
- Functional super-resolution microscopy of fibers and polymers: convergence of artificial and biological systems at the nanoscale.Nanoscale horizons · 2026Review
- Spatial Molecular Heterogeneity on Biofunctionalized Particles Quantified by Three-Dimensional Single-Molecule DNA-PAINT.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Imaging Ligand-Receptor Interactions at Single-Protein Resolution with DNA-PAINT.Small methods · 2025Article
- Revealing Spatial Molecular Heterogeneity of High-Density Biofunctionalized Surfaces Using DNA-PAINT.ACS applied materials & interfaces · 2024Article
- Quantification of absolute labeling efficiency at the single-protein level.Nature methods · 2024Article
- Nanoscale organization of the endogenous ASC speck.iScience · 2023Article
- Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.International journal of molecular sciences · 2023Article
- Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy.Journal of the American Chemical Society · 2023Article
- Affimers and nanobodies as molecular probes and their applications in imaging.Journal of cell science · 2022Article
- Fluorescence nanoscopy at the sub-10 nm scale.Biophysical reviews · 2021Review
- Completing the canvas: advances and challenges for DNA-PAINT super-resolution imaging.Trends in biochemical sciences · 2021Review
- Quantitative Assessment of Labeling Probes for Super-Resolution Microscopy Using Designer DNA Nanostructures.Chemphyschem : a European journal of chemical physics and physical chemistry · 2021Article
- Quantitative Imaging With DNA-PAINT for Applications in Synaptic Neuroscience.Frontiers in synaptic neuroscience · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
Improving labeling probes for state-of-the-art super-resolution microscopy is becoming of major importance. However, there is currently a lack of tools to quantitatively evaluate probe performance regarding efficiency, precision, and achievable resolution in an unbiased yet modular fashion. Herein, we introduce designer DNA origami structures combined with DNA-PAINT to overcome this issue and evaluate labeling efficiency, precision, and quantification using antibodies and nanobodies as exemplary labeling probes. Whereas current assessment of binders is mostly qualitative, e. g. based on an expected staining pattern, we herein present a quantitative analysis platform of the antigen labeling efficiency and achievable resolution, allowing researchers to choose the best performing binder. The platform can furthermore be readily adapted for discovery and precise quantification of a large variety of additional labeling probes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.