ArticleSTAR protocols2021
An optimized protocol for rapid, sensitive and robust on-bead ChIP-seq from primary cells.
Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Improved spike-in normalization clarifies the relationship between active histone modifications and transcription.Nature genetics · 2026Article
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- The Disordered Region of ASXL1 Acts as an Auto-Regulator Through Condensation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- FOXP3 expression depends on cell-type-specific cis-regulatory elements and transcription factor circuitry.Immunity · 2026Article
- Automated chromatin profiling with spa-ChIP-seq uncovers the impacts of condition variations.Genome research · 2026Article
- Protocol for double-crosslinking ChIP-seq to improve data quality and enhance detection of challenging chromatin targets.STAR protocols · 2025Article
- Refined ChIP-Seq Protocol for High-Quality Chromatin Profiling in Solid Tissues Using the Complete Genomics/MGI Sequencing Platform.Current protocols · 2025Article
- Advances in Epigenomic Sequencing and Their Applications in Cancer Diagnostics.Precision chemistry · 2025Review
- Automated chromatin profiling with spa-ChIP-seq uncovers the impacts of condition variations.bioRxiv : the preprint server for biology · 2025Article
- Article
- Unique nigral and cortical pathways implicated by epigenomic and transcriptional analyses in rotenone Parkinson's model.NPJ Parkinson's disease · 2025Article
- Cis-Regulatory Element and Transcription Factor Circuitry Required for Cell-Type Specific Expression of FOXP3.bioRxiv : the preprint server for biology · 2025Article
- Article
- The Wild West of spike-in normalization.Nature biotechnology · 2024Article
- Article
- Article
- RREB1 regulates neuronal proteostasis and the microtubule network.Science advances · 2024Article
- Discrimination of cell-intrinsic and environment-dependent effects of natural genetic variation on Kupffer cell epigenomes and transcriptomes.Nature immunology · 2023Article
- SALL1 enforces microglia-specific DNA binding and function of SMADs to establish microglia identity.Nature immunology · 2023Article
- Microglial pattern recognition via IL-33 promotes synaptic refinement in developing corticothalamic circuits in mice.The Journal of experimental medicine · 2023Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Integrative analysis of next-generation sequencing data can help understand disease mechanisms. Specifically, ChIP-seq can illuminate where transcription regulators bind to regulate transcription. A major obstacle to performing this assay on primary cells is the low numbers obtained from tissues. The extensively validated ChIP-seq protocol presented here uses small volumes and single-pot on-bead library preparation to generate diverse high-quality ChIP-seq data. This protocol allows for medium-to-high-throughput ChIP-seq of low-abundance cells and can also be applied to other mammalian cells. For complete details on the use and execution of this protocol, please refer to Brigidi et al. (2019), Carlin et al. (2018), Heinz et al. (2018), Nott et al. (2019), Sakai et al. (2019), and Seidman et al. (2020).
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Registered trials
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