Evidence map›Paper›PMID 33718091›Full record

ArticleTranslational andrology and urology2021

The effects of histone crotonylation and bromodomain protein 4 on prostate cancer cell lines.

Xiaolin Xu, Xin Zhu, Feng Liu, Wenlong Lu, Yihan Wang, Jianjun Yu

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational andrology and urology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 44 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Histone crotonylation in tumors (Review).Molecular and clinical oncology · 2025
    Review
  7. Article
  8. Review
  9. Review
  10. Article
  11. Review
  12. Review
  13. Protein crotonylation: Basic research and clinical diseases.Biochemistry and biophysics reports · 2024
    Review
  14. Article
  15. Review
  16. Review
  17. Review
  18. Crotonylation modification and its role in diseases.Frontiers in molecular biosciences · 2024
    Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Xiaolin XuDepartment of Urology, The Sixth People's Hospital South Campus Affiliated to Shanghai Jiaotong University, Shanghai, China.
Xin ZhuDepartment of Urology, The Sixth People's Hospital South Campus Affiliated to Shanghai Jiaotong University, Shanghai, China.
Feng LiuDepartment of Urology, The Sixth People's Hospital South Campus Affiliated to Shanghai Jiaotong University, Shanghai, China.
Wenlong LuDepartment of Urology, The Sixth People's Hospital South Campus Affiliated to Shanghai Jiaotong University, Shanghai, China.
Yihan WangDepartment of Urology, The Sixth People's Hospital South Campus Affiliated to Shanghai Jiaotong University, Shanghai, China.
Jianjun YuDepartment of Urology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Shanghai Sixth People's Hospital · CNZhejiang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe aims of this study were to detect the level of histone crotonylation in prostate cancer (PCa) tissues, analyze the correlations between its level and clinical stage and grade, and explore the effects of bromodomain-containing protein 4 (BRD4) inhibitors and sodium crotonate on the histone crotonylation in PCa cell lines and on the functions of PCa cells.

methodsPCa tissues from 72 patients and adjacent tissues from 7 patients were collected, and immunohistochemistry was used to measure the level of histone crotonylation. Three human PCa cell lines, PC-3, LNCaP, and C42B, were selected and treated with IC50 value of I-BET762, I-BET726, and CPI-203, respectively. Next, short hairpin RNA (shRNA) transient knockdown was used to inhibit BRD4 expression. Histone crotonylation level and the expression of acetylase were determined by Western blotting. Finally, cell proliferation, migration, and invasion were measured with Cell Counting Kit-8 assay, scratch test, and Transwell test respectively.

resultsThe level of histone crotonylation in PCa tissue was higher than that in adjacent tissues, and histone lysine crotonylation (Kcr) increased with the increasing malignancy of PCa. Treatments with I-BET762, I-BET726, and CPI-203 could inhibit the proliferation, migration, and invasion of PCa cell lines including PC-3, LNCaP, and C42B, and could also regulate the histone crotonylation and androgen receptor signaling pathways via the regulation of BRD4 expression.

conclusionsPCa is closely related to histone crotonylation. Inhibition of BRD4 expression can inhibit the proliferation, migration, and invasion of PCa cells.

Indexed as

bromodomain-containing protein 4 inhibitorshistone crotonylationProstate cancer (PCa)sodium crotonate

Identifiers

PMID33718091
PMCPMC7947446
OpenAlexW3133973143

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.