ArticleEcology and evolution2021
Optimization and validation of a cost-effective protocol for biosurveillance of invasive alien species.
Article in Ecology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Flush With Data (or) Optimizing and Validating the Efficacy of Free and Computationally Simple 16S Metabarcoding Approaches for Use in Wastewater Surveillance.Environmental microbiology · 2026Article
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- Assessment of leaf-litter invertebrate biodiversity using high throughput sequencing.PloS one · 2026Article
- StrataSeq: A Workflow for Rapid Development of Molecular Databases for Hard-To-Identify Species.Ecology and evolution · 2025Article
- The "Dry-Lab" Side of Food Authentication: Benchmark of Bioinformatic Pipelines for the Analysis of Metabarcoding Data.Foods (Basel, Switzerland) · 2024Article
- Hidden diversity: DNA metabarcoding reveals hyper-diverse benthic invertebrate communities.BMC ecology and evolution · 2023Article
- Development of Novel Early Detection Technology for Hemlock Woolly Adelgid, Adelges tsugae (Hemiptera: Adelgidae).Journal of economic entomology · 2023Article
- Article
- Article
- Mitochondrial genome sequencing, mapping, and assembly benchmarking for Culicoides species (Diptera: Ceratopogonidae).BMC genomics · 2022Article
- Real-Time On-Site Diagnosis of Quarantine Pathogens in Plant Tissues by Nanopore-Based Sequencing.Pathogens (Basel, Switzerland) · 2022Article
- Identifying, reducing, and communicating uncertainty in community science: a focus on alien species.Biological invasions · 2022Review
- Validation of an Effective Protocol forInsects · 2021Article
- Optimization and validation of a cost-effective protocol for biosurveillance of invasive alien species.Ecology and evolution · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental DNA (eDNA) metabarcoding has revolutionized biodiversity monitoring and invasive pest biosurveillance programs. The introduction of insect pests considered invasive alien species (IAS) into a non-native range poses a threat to native plant health. The early detection of IAS can allow for prompt actions by regulating authorities, thereby mitigating their impacts. In the present study, we optimized and validated a fast and cost-effective eDNA metabarcoding protocol for biosurveillance of IAS and characterization of insect and microorganism diversity. Forty-eight traps were placed, following the CFIA's annual forest insect trapping survey, at four locations in southern Ontario that are high risk for forest IAS. We collected insects and eDNA samples using Lindgren funnel traps that contained a saturated salt (NaCl) solution in the collection jar. Using cytochrome c oxidase I (COI) as a molecular marker, a modified Illumina protocol effectively identified 2,535 Barcode Index Numbers (BINs). BINs were distributed among 57 Orders and 304 Families, with the vast majority being arthropods. Two IAS (
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