Evidence map›Paper›PMID 33715625›Full record

ArticleWorld journal of surgical oncology2021

Circ-KIAA0907 inhibits the progression of oral squamous cell carcinoma by regulating the miR-96-5p/UNC13C axis.

Wenjie Dong, Lei Zhao, Shiyang Zhang, Shijie Zhang, Hongyun Si

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of surgical oncology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Wenjie DongDepartment of Stomatology, The Second Affiliated Hospital of Mudanjiang Medical College, No. 15, Dongxiaoyun Street, Aimin District, Mudanjiang, 157000, Heilongjiang Province, China.
Lei ZhaoDepartment of Stomatology, The Second Affiliated Hospital of Mudanjiang Medical College, No. 15, Dongxiaoyun Street, Aimin District, Mudanjiang, 157000, Heilongjiang Province, China.
Shiyang ZhangDepartment of Stomatology, The Second Affiliated Hospital of Mudanjiang Medical College, No. 15, Dongxiaoyun Street, Aimin District, Mudanjiang, 157000, Heilongjiang Province, China.
Shijie ZhangDepartment of General Surgery, The Second Affiliated Hospital of Mudanjiang Medical College, Mudanjiang, 157000, Heilongjiang Province, China.
Hongyun SiDepartment of Stomatology, The Second Affiliated Hospital of Mudanjiang Medical College, No. 15, Dongxiaoyun Street, Aimin District, Mudanjiang, 157000, Heilongjiang Province, China. wdlvcs@163.com.
Mudanjiang Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCircular RNA (circRNA) plays an important role in regulating cell biological function and has been shown to be involved in cancer progression, including oral squamous cell carcinoma (OSCC). Circ-KIAA0907 has been found to play an anti-cancer role in OSCC, so it is worth exploring more functions and new mechanisms of circ-KIAA0907 in OSCC progression.

methodsQuantitative real-time PCR (qRT-PCR) was used to detect the expression of circ-KIAA0907, microRNA (miR)-96-5p, and unc-13 homolog C (UNC13C). Transwell assay, flow cytometry, and colony formation assay were employed to measure the migration, invasion, apoptosis, and radiosensitivity of cells. Besides, glucose uptake, lactate production, and extracellular acidification rate (ECAR) were determined to evaluate the glycolysis ability of cells. Dual-luciferase reporter assay and RIP assay were performed to confirm the interactions among circ-KIAA0907, miR-96-5p, and UNC13C. And RNA pull-down assay was used to verify the binding degree of miR-96-5p to its targets. Moreover, UNC13C protein level was examined using western blot (WB) analysis. OSCC xenograft models were constructed to perform in vivo experiments.

resultsCirc-KIAA0907 was a stability circRNA with lowly expression in OSCC. Overexpressed circ-KIAA0907 could inhibit migration, invasion, and glycolysis, while promoting apoptosis and radiosensitivity in OSCC cells. In the terms of mechanism, circ-KIAA0907 could sponge miR-96-5p to regulate UNC13C expression. MiR-96-5p overexpression could reverse the inhibitory effect of circ-KIAA0907 on OSCC progression, and UNC13C knockdown also could overturn the suppressive effect of miR-96-5p inhibitor on OSCC progression. Animal experiments revealed that circ-KIAA0907 could reduce the tumor growth of OSCC by regulating the miR-96-5p/UNC13C axis.

conclusionOur study suggests that circ-KIAA0907 restrains OSCC progression via the miR-96-5p/UNC13C axis, indicating that it may be a potential target for OSCC treatment.

Indexed as

Carcinoma, Squamous CellHead and Neck NeoplasmsMicroRNAsMouth NeoplasmsAnimalsCell ProliferationNerve Tissue ProteinsPrognosisRNA, CircularSquamous Cell Carcinoma of Head and NeckMicroRNAsMIRN96 microRNA, humanNerve Tissue ProteinsRNA, CircularCirc-KIAA0907MiR-96-5pOral squamous cell carcinomaUNC13C

Identifiers

PMID33715625
PMCPMC7962272
OpenAlexW3139424353

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.