ArticleInternational endodontic journal2021
Histology-based profile of inflammatory mediators in experimentally induced pulpitis in a rat model: screening for possible biomarkers.
Article in International endodontic journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Salvianolic acid B functionalized injectable GelMA hydrogel for pulpitis in a vital pulp therapy: a dual anti-inflammatory property and enhanced reparative dentinogenesis activity material.BMC oral health · 2026Article
- Effect of TheraCal PT and Biodentine on inflammatory cell infiltration and hard tissue formation after pulpotomy in inflamed or healthy rat molars.Clinical oral investigations · 2025Article
- Application and mechanism study of EMD-Gel composite scaffold in dental pulp tissue repair.Frontiers in bioengineering and biotechnology · 2025Article
- A ROS-responsive hydrogel incorporated with dental follicle stem cell-derived small extracellular vesicles promotes dental pulp repair by ameliorating oxidative stress.Bioactive materials · 2024Article
- Chemokine‑like receptor 1‑positive cells are present in the odontoblast layer in tooth tissue in rats and humans.Experimental and therapeutic medicine · 2024Article
- Article
- Concentrated growth factor combined with iRoot BP Plus promotes inflamed pulp repair: an in vitro and in vivo study.BMC oral health · 2023Article
- Small extracellular vesicles derived from hypoxic preconditioned dental pulp stem cells ameliorate inflammatory osteolysis by modulating macrophage polarization and osteoclastogenesis.Bioactive materials · 2023Article
- Expression of Toll-like receptor 2, Dectin-1, and Osteopontin in murine model of pulpitis.Clinical oral investigations · 2023Article
- Analysis of circRNAs profile in TNF-α treated DPSC.BMC oral health · 2022Article
- Histology-based profile of inflammatory mediators in experimentally induced pulpitis in a rat model: screening for possible biomarkers.International endodontic journal · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
aimTo profile molecular changes in lipopolysaccharide (LPS)-induced experimental pulpitis in a rat model and explore the feasibility of a molecular-based diagnostic strategy for pulpitis. METHODOLOGY: Seventy-three maxillary incisors of Sprague-Dawley rats were used to establish pulpitis models with LPS. Inflammatory grading was performed in four equal sections of the pulp divided from the injured site to the root apex. An antibody array was used to compare the expression of 67 molecules between control pulp and inflamed pulp 12 and 72 h after LPS application. The levels of differentially expressed molecules in the control and inflamed pulp (collected at 3, 6, 9, 12, 24 and 72 h after LPS treatment) were examined via ELISA, and correlations between inflammatory scores and molecule expression were assessed. The molecule distributions in the pulp were investigated by immunofluorescence staining. Data were analysed with paired t-test, one-way anova, Kruskal-Wallis tests, and Spearman's and Pearson's correlations with significance set at P < 0.05.
resultsPolymorphonuclear neutrophils were observed in the injured site 3 h after LPS stimulation. Inflammatory infiltration peaked at 12 h and was limited to the injured site with osteodentine deposition at 72 h. Thirteen molecules were significantly differentially expressed between the control and LPS-injured pulp. ELISA validated that tissue inhibitor of metalloproteinase-1 (TIMP-1) expression dramatically peaked at 12 h (compared with other time points, P < 0.05) and returned to baseline at 72 h. The TIMP-1 concentration was strongly correlated with inflammation severity in the apical three-quarters of the pulp, and the strongest correlation was found in the lower-middle quarter (r = 0.786, P < 0.001). Immunofluorescence staining revealed that in the apical three-quarters of the pulp, TIMP-1 expression was significantly higher in the 12 h group than in the control and 3, 6, 24 and 72 h groups (P < 0.01).
conclusionThis study provides a molecular profile of LPS-induced pulpitis in a rat model. TIMP-1 had a strong positive correlation with the severity of dental pulp inflammation, verifying the feasibility of applying biomarkers to identify specific pathological conditions in pulpitis.
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