ArticleThe Journal of comparative neurology2021
Efferent projections of CGRP/Calca-expressing parabrachial neurons in mice.
Article in The Journal of comparative neurology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 70 citations in OpenAlex.
- Hypothalamic Glutamatergic/Orexinergic Neurons Attenuate Fentanyl-Induced Respiratory Depression via Medullary Pre-BöTzinger Complex Pathways.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Molecular Ontology Predicts Output Connections From the Nucleus of the Solitary Tract.The Journal of comparative neurology · 2026Article
- ParabrachialProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Intersectional expression ofAmerican journal of physiology. Regulatory, integrative and comparative physiology · 2026Article
- The pyrogenic mediator prostaglandin EThe Journal of physiology · 2026Article
- The Role of the Ventral Tegmental Area in Reward and Addiction: A Comprehensive Review.Neurosciences (Riyadh, Saudi Arabia) · 2026Review
- Multimodal Breathing Control: Pontomedullary Mechanisms and Current Perspectives.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- ParabrachialeNeuro · 2025Article
- Distinctive biochemical properties of the μ-opioid receptor-corticotropin- releasing factor CRFPharmacological research · 2025Article
- Calcitonin Gene-Related Peptide (CGRP)-Expressing Neurons in the External Lateral Parabrachial Area Regulate Pain-Induced Sleep Disturbances.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- ParabrachialiScience · 2025Article
- The posterior capsular central amygdala showing synaptic coactivation with nociplastic pain-associated parabrachial neurons in mice.iScience · 2025Article
- Serotonin neurons are necessary for tonic sodium intake inhibition.American journal of physiology. Regulatory, integrative and comparative physiology · 2025Article
- Quantitative characterization of cell niches in spatially resolved omics data.Nature genetics · 2025Article
- Article
- Afferent Projections to the Calca/CGRP-Expressing Parabrachial Neurons in Mice.The Journal of comparative neurology · 2025Article
- Characterization of direct Purkinje cell outputs to the brainstem.bioRxiv : the preprint server for biology · 2024Article
- Control of sodium appetite by hindbrain aldosterone-sensitive neurons.Molecular and cellular endocrinology · 2024Review
- Article
- Morphological Tracing and Functional Identification of Monosynaptic Connections in the Brain: A Comprehensive Guide.Neuroscience bulletin · 2024Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The parabrachial nucleus (PB) is composed of glutamatergic neurons at the midbrain-hindbrain junction. These neurons form many subpopulations, one of which expresses Calca, which encodes the neuropeptide calcitonin gene-related peptide (CGRP). This Calca-expressing subpopulation has been implicated in a variety of homeostatic functions, but the overall distribution of Calca-expressing neurons in this region remains unclear. Also, while previous studies in rats and mice have identified output projections from CGRP-immunoreactive or Calca-expressing neurons, we lack a comprehensive understanding of their efferent projections. We began by identifying neurons with Calca mRNA and CGRP immunoreactivity in and around the PB, including populations in the locus coeruleus and motor trigeminal nucleus. Calca-expressing neurons in the PB prominently express the mu opioid receptor (Oprm1) and are distinct from neighboring neurons that express Foxp2 and Pdyn. Next, we used Cre-dependent anterograde tracing with synaptophysin-mCherry to map the efferent projections of these neurons. Calca-expressing PB neurons heavily target subregions of the amygdala, bed nucleus of the stria terminalis, basal forebrain, thalamic intralaminar and ventral posterior parvicellular nuclei, and hindbrain, in different patterns depending on the injection site location within the PB region. Retrograde axonal tracing revealed that the previously unreported hindbrain projections arise from a rostral-ventral subset of CGRP/Calca neurons. Finally, we show that these efferent projections of Calca-expressing neurons are distinct from those of neighboring PB neurons that express Pdyn. This information provides a detailed neuroanatomical framework for interpreting experimental work involving CGRP/Calca-expressing neurons and opioid action in the PB region.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.