ArticleNitric oxide : biology and chemistry2021
Control of rat muscle nitrate levels after perturbation of steady state dietary nitrate intake.
Article in Nitric oxide : biology and chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- The compartmentalization model of nitrate homeostasis: Role of the salivary glands, skeletal muscle, and liver.EXCLI journal · 2026Review
- Dietary nitrate supplementation mitigates age-related changes at the neuromuscular junction in mice.The Journal of physiology · 2026Article
- Effect of pre-exercise dietary nitrate on skeletal muscle blood flow in a rat model of pulmonary hypertension.American journal of physiology. Regulatory, integrative and comparative physiology · 2025Article
- Long-term nitrate administration modulates sialin gene expression in the main tissues of male Wistar rats with type 2 diabetes.EXCLI journal · 2025Article
- Type 2 diabetes-related sarcopenia: role of nitric oxide.Nutrition & metabolism · 2024Review
- Skeletal Muscle, Skin, and Bone as Three Major Nitrate Reservoirs in Mammals: Chemiluminescence andNutrients · 2024Article
- Exercise Training Decreases Nitrite Concentration in the Heart and Locomotory Muscles of Rats Without Changing the Muscle Nitrate Content.Journal of the American Heart Association · 2024Article
- High nitrate levels in skeletal muscle contribute to nitric oxide generation via a nitrate/nitrite reductive pathway in mice that lack the nNOS enzyme.Frontiers in physiology · 2024Article
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- Altered sialin mRNA gene expression in type 2 diabetic male Wistar rats: implications for nitric oxide deficiency.Scientific reports · 2023Article
- Distribution of dietary nitrate and its metabolites in rat tissues afterScientific reports · 2023Article
- Time course of human skeletal muscle nitrate and nitrite concentration changes following dietary nitrate ingestion.Nitric oxide : biology and chemistry · 2022Article
- Nitrite Concentration in the Striated Muscles Is Reversely Related to Myoglobin and Mitochondrial Proteins Content in Rats.International journal of molecular sciences · 2022Article
- Quantitative aspects of nitric oxide production from nitrate and nitrite.EXCLI journal · 2022Review
- Human and rodent red blood cells do not demonstrate xanthine oxidase activity or XO-catalyzed nitrite reduction to NO.Free radical biology & medicine · 2021Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
The roles of nitrate and nitrite ions as nitric oxide (NO) sources in mammals, complementing NOS enzymes, have recently been the focus of much research. We previously reported that rat skeletal muscle serves as a nitrate reservoir, with the amount of stored nitrate being highly dependent on dietary nitrate availability, as well as its synthesis by NOS1 enzymes and its subsequent utilization. We showed that at conditions of increased NO need, this nitrate reservoir is used in situ to generate nitrite and NO, at least in part via the nitrate reductase activity of xanthine oxidoreductase (XOR). We now further investigate the dynamics of nitrate/nitrite fluxes in rat skeletal muscle after first increasing nitrate levels in drinking water and then returning to the original intake level. Nitrate/nitrite levels were analyzed in liver, blood and several skeletal muscle samples, and expression of proteins involved in nitrate metabolism and transport were also measured. Increased nitrate supply elevated nitrate and nitrite levels in all measured tissues. Surprisingly, after high nitrate diet termination, levels of both ions in liver and all muscle samples first declined to lower levels than the original baseline. During the course of the overall experiment there was a gradual increase of XOR expression in muscle tissue, which likely led to enhanced nitrate to nitrite reduction. We also noted differences in basal levels of nitrate in the different types of muscles. These findings suggest complex control of muscle nitrate levels, perhaps with multiple processes to preserve its intracellular levels.
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