ArticleJournal of structural biology2021
Rapid tilt-series method for cryo-electron tomography: Characterizing stage behavior during FISE acquisition.
Article in Journal of structural biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Article
- Advances in automation for cryo-electron tomography data collection.Current opinion in structural biology · 2025Review
- Electron Cryo-Tomography Reveals thebioRxiv : the preprint server for biology · 2025Article
- Structural biology inside multicellular specimens using electron cryotomography.Quarterly reviews of biophysics · 2025Review
- Cryogenic Electron Microscopy of Rift Valley Fever Virus.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Combining per-particle cryo-ET and cryo-EM single particle analysis to elucidate heterogeneous DNA-protein organization.Current opinion in structural biology · 2024Review
- Rapid Synthesis of Cryo-ET Data for Training Deep Learning Models.bioRxiv : the preprint server for biology · 2023Article
- Structure and Function of the Dot/Icm T4SS.bioRxiv : the preprint server for biology · 2023Article
- Structural basis of a transcription pre-initiation complex on a divergent promoter.Molecular cell · 2023Article
- Montage electron tomography of vitrified specimens.Journal of structural biology · 2022Article
- A feature-guided, focused 3D signal permutation method for subtomogram averaging.Journal of structural biology · 2022Article
- How advances in cryo-electron tomography have contributed to our current view of bacterial cell biology.Journal of structural biology: X · 2022Article
- Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows.Journal of visualized experiments : JoVE · 2021Article
- Programmed Flagellar Ejection in Caulobacter crescentus Leaves PL-subcomplexes.Journal of molecular biology · 2021Article
- Challenges in solving structures from radiation-damaged tomograms of protein nanocrystals assessed by simulation.Acta crystallographica. Section D, Structural biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We and others recently developed rapid tilt-series acquisition methods for cryo-electron tomography on a Titan Krios G3i equipped with a single axis holder and a K-series direct electron detector and showed that one of these, the fast-incremental single exposure (FISE) method, significantly accelerates tilt-series acquisition when compared to traditional methods while preserving the quality of the images. Here, we characterize the behavior of our single axis holder in detail during a FISE experiment to optimally balance data quality with speed. We explain our methodology in detail so others can characterize their own stages, and conclude with recommendations for projects with different resolution goals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.