ArticleThe Plant cell2021
Long-read sequence assembly: a technical evaluation in barley.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 237 papers, 1 of them a synthesis that pooled it.
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Who cites it
237 citing papers in PubMed, 1 synthesis or guideline pooled it, 405 citations in OpenAlex.
- New loci and candidate genes in spring two-rowed barley detected through meta-analysis of a field trial European network.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Pooled it
- A haplotype-based approach to explore genotype-by-environment interactions for barley yield.The plant genome · 2026Article
- Genome-wide association analysis of resistance to scald in an adapted multiparent winter malting barley population.The plant genome · 2026Article
- Auxin-related regulation of barley floral organ fate: genetic and phenotypic insights from tweaky spike mutants.The New phytologist · 2026Article
- Leaf variegation in a barley EMS mutant is controlled by two epistatic mutations.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- A multi-omic dissection of spatial hierarchy-dependent grain development in barley (Hordeum vulgare L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- The role of methylation and structural variants in shaping the recombination landscape of barley.The Plant journal : for cell and molecular biology · 2026Article
- Discovering common and population-specific QTLs for leaf rust resistance in different Barley populations.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Genome-wide identification and expression analysis of Aspartic proteases in Hordeum vulagare L. reveals candidates involved in salt and drought tolerance.Protoplasma · 2026Article
- Chromosome-scale assembly of wheat cultivar Sumai 3, a major germplasm source for Fusarium head blight resistance.The plant genome · 2026Article
- Genome-Wide Characterization of Genetic Diversity and Population Structure in a Kazakhstani Two-Row Spring Barley Breeding Panel.International journal of molecular sciences · 2026Article
- Unveiling centromeric retrotransposon dynamics through a high-quality rye genome assembly.Nature communications · 2026Article
- Transcriptome and Hormone Regulations Shape Drought Stress-Dependent Fusarium Head Blight Susceptibility in Different Barley Genotypes.Plant, cell & environment · 2026Article
- Spatial Regulation of Silicon Accumulation in Peduncle Confers Sheathed Spike in Barley.Plant biotechnology journal · 2026Article
- Integrated Comparative Genomics and Population Genomics Reveal the Genomic and Epigenomic Basis of Barley (Plants (Basel, Switzerland) · 2026Article
- Nanopore sequencing reveals coordinated host and microbiome responses across barley genotypes.BMC biology · 2026Article
- Golden Promise-rapid, a fast-cycling and transformable barley genotype.Journal of experimental botany · 2026Article
- Resistance to the oat leaf blotch pathogen (Pyrenophora avenae) is delimited to a region of chromosome 1D that harbors a candidate resistance kinase fusion protein.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- The effect of foliar chitosan application on fusarium head blight in barley is highly genotype dependent.BMC plant biology · 2026Article
- COMPOSITUM 2/ WHEAT FRIZZY PANICLE dosage dependently regulates inflorescence and root architecture in Triticeae cereals.The Plant journal : for cell and molecular biology · 2026Article
177 more citing papers are in PubMed but not listed here.
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Authors and funding
22 authors at 8 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequence assembly of large and repeat-rich plant genomes has been challenging, requiring substantial computational resources and often several complementary sequence assembly and genome mapping approaches. The recent development of fast and accurate long-read sequencing by circular consensus sequencing (CCS) on the PacBio platform may greatly increase the scope of plant pan-genome projects. Here, we compare current long-read sequencing platforms regarding their ability to rapidly generate contiguous sequence assemblies in pan-genome studies of barley (Hordeum vulgare). Most long-read assemblies are clearly superior to the current barley reference sequence based on short-reads. Assemblies derived from accurate long reads excel in most metrics, but the CCS approach was the most cost-effective strategy for assembling tens of barley genomes. A downsampling analysis indicated that 20-fold CCS coverage can yield very good sequence assemblies, while even five-fold CCS data may capture the complete sequence of most genes. We present an updated reference genome assembly for barley with near-complete representation of the repeat-rich intergenic space. Long-read assembly can underpin the construction of accurate and complete sequences of multiple genomes of a species to build pan-genome infrastructures in Triticeae crops and their wild relatives.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.