ArticleThe Plant cell2021
ERF4 and MYB52 transcription factors play antagonistic roles in regulating homogalacturonan de-methylesterification in Arabidopsis seed coat mucilage.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 69 citations in OpenAlex.
- A Natural LTR Retrotransposon Insertion in the Promoter of GhNAC140-Dt Boosts Cotton Lint Yield.Plant biotechnology journal · 2026Article
- Advances in the functional research of pectin methylesterases in Arabidopsis thaliana.BMC plant biology · 2026Review
- Pectin Metabolism Influences Phloem Architecture and Flowering Time in Arabidopsis Thaliana.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Transcriptomic analysis of Camellia japonica to scale insects infestation and functional characterization of pectin methylesterase gene CjPME28 and polygalacturonase gene CjPG1.Plant cell reports · 2025Article
- Mucilicious methods: Navigating the tools developed to Arabidopsis Seed Coat Mucilage analysis.Cell surface (Amsterdam, Netherlands) · 2025Review
- Spatiotemporal transcriptome and metabolome landscapes of cotton somatic embryos.Nature communications · 2025Article
- A genome-wide association study identifies candidate genes for target leaf spot disease resistance in adult cucumber (Frontiers in plant science · 2025Article
- Article
- OsMYB1 antagonizes OsSPL14 to mediate rice resistance to brown planthopper and Xanthomonas oryzae pv. oryzae.Plant cell reports · 2024Article
- MYB52 negatively regulates ADF9-meditated actin filament bundling in Arabidopsis pavement cell morphogenesis.Journal of integrative plant biology · 2024Article
- The transcription factors ZAT5 and BLH2/4 regulate homogalacturonan demethylesterification in Arabidopsis seed coat mucilage.The Plant cell · 2024Article
- Single-Cell Transcriptome Atlas and Regulatory Dynamics in Developing Cotton Anthers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Time-Course Transcriptome Analysis ofInternational journal of molecular sciences · 2023Article
- Functional investigation of five R2R3-MYB transcription factors associated with wood development in Eucalyptus using DAP-seq-ML.Plant molecular biology · 2023Article
- MYB-bHLH-TTG1 in a Multi-tiered Pathway Regulates Arabidopsis Seed Coat Mucilage Biosynthesis Genes Including PECTIN METHYLESTERASE INHIBITOR14 Required for Homogalacturonan Demethylesterification.Plant & cell physiology · 2023Article
- PECTIN METHYLESTERASE INHIBITOR18 functions in stomatal dynamics and stomatal dimension.Plant physiology · 2023Article
- Downregulation ofFrontiers in plant science · 2023Article
- Article
- Identification of novel candidate loci and genes for seed vigor-related traits in upland cotton (Frontiers in plant science · 2023Article
- Integrated physicochemical, hormonal, and transcriptomic analysis reveals the underlying mechanism of callus formation inFrontiers in plant science · 2023Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homogalacturonan (HG), a component of pectin, is synthesized in the Golgi apparatus in its fully methylesterified form. It is then secreted into the apoplast where it is typically de-methylesterified by pectin methylesterases (PME). Secretion and de-esterification are critical for normal pectin function, yet the underlying transcriptional regulation mechanisms remain largely unknown. Here, we uncovered a mechanism that fine-tunes the degree of HG de-methylesterification (DM) in the mucilage that surrounds Arabidopsis thaliana seeds. We demonstrate that the APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor (TF) ERF4 is a transcriptional repressor that positively regulates HG DM. ERF4 expression is confined to epidermal cells in the early stages of seed coat development. The adhesiveness of the erf4 mutant mucilage was decreased as a result of an increased DM caused by a decrease in PME activity. Molecular and genetic analyses revealed that ERF4 positively regulates HG DM by suppressing the expression of three PME INHIBITOR genes (PMEIs) and SUBTILISIN-LIKE SERINE PROTEASE 1.7 (SBT1.7). ERF4 shares common targets with the TF MYB52, which also regulates pectin DM. Nevertheless, the erf4-2 myb52 double mutant seeds have a wild-type mucilage phenotype. We provide evidence that ERF4 and MYB52 regulate downstream gene expression in an opposite manner by antagonizing each other's DNA-binding ability through a physical interaction. Together, our findings reveal that pectin DM in the seed coat is fine-tuned by an ERF4-MYB52 transcriptional complex.
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