Evidence map›Paper›PMID 33709000›Full record

ArticleJournal of diabetes research2021

miR-126 Mimic Counteracts the Increased Secretion of VEGF-A Induced by High Glucose in ARPE-19 Cells.

Roberta Sanguineti, Alessandra Puddu, Massimo Nicolò, Carlo Enrico Traverso, Renzo Cordera, Giorgio L Viviani, Davide Maggi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of diabetes research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. MicroRNA-126 (MiR-126): key roles in related diseases.Journal of physiology and biochemistry · 2024
    Review
  12. Article
  13. Review
  14. High Glucose Impairs Expression and Activation of MerTK in ARPE-19 Cells.International journal of molecular sciences · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Roberta SanguinetiDepartment of Internal Medicine and Medical Specialties, Viale Benedetto XV XV, Genova, Italy.ORCID https://orcid.org/0000-0002-3130-9372
Alessandra PudduDepartment of Internal Medicine and Medical Specialties, Viale Benedetto XV XV, Genova, Italy.ORCID https://orcid.org/0000-0002-9084-2636
Massimo NicolòDepartment of Neuroscience, Ophthalmology and Genetics, Viale Benedetto, Genova, Italy.ORCID https://orcid.org/0000-0002-7824-3091
Carlo Enrico TraversoDepartment of Neuroscience, Ophthalmology and Genetics, Viale Benedetto, Genova, Italy.
Renzo CorderaDepartment of Internal Medicine and Medical Specialties, Viale Benedetto XV XV, Genova, Italy.ORCID https://orcid.org/0000-0002-5280-9691
Giorgio L VivianiDepartment of Internal Medicine and Medical Specialties, Viale Benedetto XV XV, Genova, Italy.ORCID https://orcid.org/0000-0002-5599-516X
Davide MaggiDepartment of Internal Medicine and Medical Specialties, Viale Benedetto XV XV, Genova, Italy.ORCID https://orcid.org/0000-0003-3928-1295

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vascular endothelial growth factor-A (VEGF-A) has a pathologic role in microvascular diabetic complication, such as diabetic retinopathy (DR). miR-126 plays an important role in vascular development and angiogenesis by regulating the expression of VEGF-A. Since levels of miR-126 have been found downregulated in diabetes, this study is aimed at investigating whether hyperglycemia affects expression of miR-126 in a retinal pigment epithelium cell line. ARPE-19 cells were transfected with miR-126 inhibitor or with miR-126 mimic and the respective scramble negative control. After 24 hours, medium was replaced and cells were cultured for 24 hours in normal (CTR) or diabetic condition (HG). Then, we analyzed mRNA levels of miR-126, VEGF-A, PI3KR2, and SPRED1. We also evaluated protein amount of HIF-1

Indexed as

Adaptor Proteins, Signal TransducingCell LineClass Ia Phosphatidylinositol 3-KinaseDiabetic RetinopathyGlucoseHumansHypoxia-Inducible Factor 1, alpha SubunitMicroRNAsOligonucleotidesRetinal NeovascularizationRetinal Pigment EpitheliumSignal TransductionUp-RegulationVascular Endothelial Growth Factor AAdaptor Proteins, Signal TransducingClass Ia Phosphatidylinositol 3-KinaseGlucoseHIF1A protein, humanHypoxia-Inducible Factor 1, alpha SubunitMicroRNAsMIRN126 microRNA, humanOligonucleotidesSPRED1 protein, humanVascular Endothelial Growth Factor AVEGFA protein, human

Identifiers

PMID33709000
PMCPMC7932804
OpenAlexW3129248918

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.