ArticleAnnals of translational medicine2021
MiR-4729 regulates TIE1 mRNA m6A modification and angiogenesis in hemorrhoids by targeting METTL14.
Article in Annals of translational medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 22 citations in OpenAlex.
- mBiomolecules · 2026Review
- Limited Predictive Value of NLR, PLR and SII in Thrombosed Hemorrhoids: A Real-World Analysis.Life (Basel, Switzerland) · 2026Article
- Differences in heart DNA methylation between sudden infant death syndrome and other sudden deaths.Clinical epigenetics · 2026Article
- Curcumin attenuates the progression of hemorrhoids through the inhibition of angiogenesis via miR-190a-5p/TGFBR2.Scientific reports · 2026Article
- Smad2/3 Regulates IL-6R m6A Methylation through METTL3 to Influence Inflammatory Responses in Hemorrhoidal Disease.Journal of innate immunity · 2026Article
- ADAP-METTL3 modulates the inflammatory responses of macrophages via mCell death & disease · 2025Article
- How Can Molecules Induce Hemorrhoids? The Role of Genetics and Epigenetics in Hemorrhoidal Disease.International journal of molecular sciences · 2025Review
- Recent Advances in the Mutual Regulation of m6A Modification and Non-Coding RNAs in Atherosclerosis.International journal of general medicine · 2025Review
- PKG1 promotes the HIV-induced proliferation, migration, and fibrosis of vascular smooth muscle cells of hemorrhoids.International journal of colorectal disease · 2024Article
- The role of m6A in angiogenesis and vascular diseases.iScience · 2024Review
- Distinct mRNA expression profiles and miRNA regulators of the PI3K/AKT/mTOR pathway in breast cancer: insights into tumor progression and therapeutic targets.Frontiers in oncology · 2024Article
- METTL16 suppressed the proliferation and cisplatin-chemoresistance of bladder cancer by degrading PMEPA1 mRNA in a m6A manner through autophagy pathway.International journal of biological sciences · 2024Article
- Identification of the microRNA alterations in extracellular vesicles derived from human haemorrhoids.Experimental physiology · 2023Article
- RNA modifications in cardiovascular health and disease.Nature reviews. Cardiology · 2023Review
- Traditional Chinese Medicine in the treatment of hemorrhoids-a review of preparations used and their mechanism of action.Frontiers in pharmacology · 2023Review
- Research Progress for RNA Modifications in Physiological and Pathological Angiogenesis.Frontiers in genetics · 2022Review
- Insights into roles of METTL14 in tumors.Cell proliferation · 2022Review
- N6-Methyladenosine-Sculpted Regulatory Landscape of Noncoding RNA.Frontiers in oncology · 2021Review
- Novel Insights Into the Potential Mechanisms of N6-Methyladenosine RNA Modification on Sepsis-Induced Cardiovascular Dysfunction: An Update Summary on Direct and Indirect Evidences.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHemorrhoids are a frequently-occurring disease of the anorectal system that is often accompanied by vascular hyperplasia and edema. A METTL14-mediated RNA N-6 methyladenosine (m6A) modification can improve mRNA stability and increase its transcriptional and translational activities, closely related to the occurrence of many diseases.
methodsWestern blot, qPCR, and immunofluorescence staining were used to detect the levels of gene and protein expression. Haematoxylin and eosin staining was used for histopathological examination. RNA immunoprecipitation-PCR and RNA dot blotting were used to detect mRNA m6A modification.
resultsObvious signs of angiogenesis (CD31+/vWF+) were identified in the hemorrhoids. High levels of METTL14 expression on vascular endothelial cells (CD31+) suggested that angiogenesis was accompanied by differential modification of m6A RNA. It was subsequently found that the level of miR-4729 expression was significantly decreased in hemorrhoid tissues. The luciferase reporter enzyme assay results suggested that miR-4729 silenced its expression by targeting the 3'UTR of METTL14 mRNA. MiR-4729 overexpression in human umbilical vein endothelial cells (HUVECs) inhibited the proliferation and migration of HUVECs
conclusionsOur findings confirmed that the down-regulation of miR-4729 in hemorrhoid vascular endothelial cells was one of the main reasons for vascular proliferation. The overexpression of miR-4729 in vascular endothelial cells decreased the global mRNA methylation and TIE1 mRNA 3'UTR-specific site methylation by silencing METTL14 expression, reducing TIE1 mRNA stability, down-regulating the TIE1/VEGFA signal molecular loop expression, and weakening angiogenesis ability.
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