Evidence map›Paper›PMID 33705865›Full record

ArticleAntiviral research2021

Identification of filovirus entry inhibitors targeting the endosomal receptor NPC1 binding site.

Leah Liu Wang, Nicholas Palermo, Leslie Estrada, Colton Thompson, J J Patten, Manu Anantpadma, Robert A Davey, Shi-Hua Xiang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antiviral research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Leah Liu WangSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA; Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Nicholas PalermoComputational Chemistry Core Facility, VCR Cores, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Leslie EstradaNebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Colton ThompsonNebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
J J PattenNational Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, 0211, USA.
Manu AnantpadmaNational Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, 0211, USA.
Robert A DaveyNational Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, 0211, USA.
Shi-Hua XiangSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA; Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA. Electronic address: sxiang2@unl.edu.
University of Nebraska–Lincoln · USBoston University · USUniversity of Nebraska Medical Center · US

Funding

Regulatory Compliance CoreUC7AI095321 · NIAID · BOSTON UNIVERSITY MEDICAL CAMPUS · PI Adam Carter · 2014 to 2026
$159.3M
Virology BSL4 CoreP01AI120943 · NIAID · WASHINGTON UNIVERSITY · PI Gaya K. Amarasinghe · 2016 to 2026
$30.2M
Mucosal Delivery and Retention of Ebola Inhibitor Scytovirin Using LactobacillusR21AI126299 · NIAID · UNIVERSITY OF NEBRASKA LINCOLN · PI XIANG, SHI-HUA · 2017 to 2018
$453k
NIAID NIH HHS P01 AI120943NIAID NIH HHS R21 AI126299NIAID NIH HHS UC7 AI095321
6 · The paper itself

Abstract

Filoviruses, mainly consisting of Ebola viruses (EBOV) and Marburg viruses (MARV), are enveloped negative-strand RNA viruses which can infect humans to cause severe hemorrhagic fevers and outbreaks with high mortality rates. The filovirus infection is mediated by the interaction of viral envelope glycoprotein (GP) and the human endosomal receptor Niemann-Pick C1 (NPC1). Blocking this interaction will prevent the infection. Therefore, we utilized an In silico screening approach to conduct virtual compound screening against the NPC1 receptor-binding site (RBS). Twenty-six top-hit compounds were purchased and evaluated by in vitro cell based inhibition assays against pseudotyped or replication-competent filoviruses. Two classes (A and U) of compounds were identified to have potent inhibitory activity against both Ebola and Marburg viruses. The IC

Indexed as

Antiviral AgentsBinding SitesCell SurvivalDrug DiscoveryEbolavirusFiloviridae InfectionsHeLa CellsHost Microbial InteractionsHumansInhibitory Concentration 50MarburgvirusMolecular Docking SimulationNiemann-Pick C1 ProteinProtein BindingReceptors, VirusViral Envelope ProteinsAntiviral AgentsNiemann-Pick C1 ProteinNPC1 protein, humanReceptors, VirusViral Envelope ProteinsEntry inhibitorsEnvelope glycoprotein (GP)FilovirusIn silico screeningNiemann-pick C1 (NPC1)Receptor binding site (RBS)

Identifiers

PMID33705865
PMCPMC8088776
OpenAlexW3134357226

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.