Evidence map›Paper›PMID 33704759›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing Escherichia coli-Host Interactions.

Karol Dokladny, Julie G In, James Kaper, Olga Kovbasnjuk

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
4.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Karol DokladnyDivision of Gastroenterology, Department of Internal Medicine, University of New Mexico Health Sciences Center, University of New Mexico School of Medicine, Albuquerque, NM, USA.
Julie G InDivision of Gastroenterology, Department of Internal Medicine, University of New Mexico Health Sciences Center, University of New Mexico School of Medicine, Albuquerque, NM, USA.
James KaperDepartment of Microbiology & Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.
Olga KovbasnjukDivision of Gastroenterology, Department of Internal Medicine, University of New Mexico Health Sciences Center, University of New Mexico School of Medicine, Albuquerque, NM, USA. OKovbasnjuk@salud.unm.edu.
University of New Mexico · USUniversity of Maryland, Baltimore · US

Funding

Project 3 - Human colonoids as a model for the pathobiology of EHECP01AI125181 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI KAPER, JAMES B · 2016 to 2025
$18.6M
A Microphysiological System for Kidney Disease Modeling and Drug Efficacy TestingUG3TR002158 · NCATS · UNIVERSITY OF WASHINGTON · PI HIMMELFARB, JONATHAN · 2017 to 2018
$2.9M
Human Enteroids as a Model of Host-EHEC InteractionsK01DK106323 · NIDDK · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI IN, JULIE GOEUN · 2016 to 2020
$711k
NCATS NIH HHS UG3 TR002158NIDDK NIH HHS K01 DK106323
6 · The paper itself

Abstract

Human intestinal organoid cultures established from crypt-derived stem cells truly revolutionized our approach to study intestinal epithelial physiology and pathologies as they can be propagated indefinitely and preserve the genetic signature of the donor and the gut segment specificity in culture. Here we describe human stem cell-derived colonoid monolayers as a reliable and reproducible model to study Shiga toxin-producing Escherichia coli (STEC) infection and STEC-caused pathologies of the whole colonic epithelium comprising a mixture of colonocytes, goblet, enteroendocrine, and other rare cells present in human colonic epithelial tissue.

Indexed as

ColonEpithelial CellsHost-Pathogen InteractionsIntestinal MucosaModels, BiologicalEscherichia coli InfectionsHumansShiga-Toxigenic Escherichia coliColonoid monolayersHuman intestinal organoidsShiga toxinSTECStem cells

Identifiers

PMID33704759
OpenAlexW3133618891

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.