Evidence map›Paper›PMID 33704748›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Shiga Toxin-Producing E. coli in Animals: Detection, Characterization, and Virulence Assessment.

Stefanie A Barth, Rolf Bauerfeind, Christian Berens, Christian Menge

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. EnterohemorrhagicInternational journal of molecular sciences · 2022
    Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Stefanie A BarthFriedrich-Loeffler-Institut/Federal Research Institute for Animal Health, Institute of Molecular Pathogenesis, Jena, Germany.
Rolf BauerfeindInstitute for Hygiene and Infectious Diseases of Animals, Justus Liebig University Gießen, Gießen, Germany.
Christian BerensFriedrich-Loeffler-Institut/Federal Research Institute for Animal Health, Institute of Molecular Pathogenesis, Jena, Germany.
Christian MengeFriedrich-Loeffler-Institut/Federal Research Institute for Animal Health, Institute of Molecular Pathogenesis, Jena, Germany. christian.menge@fli.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cattle and other ruminants are primary reservoirs for Shiga toxin-producing Escherichia coli (STEC) strains which have a highly variable, but unpredictable, pathogenic potential for humans. Domestic swine can carry and shed STEC, but only STEC strains producing the Shiga toxin (Stx) 2e variant and causing edema disease in piglets are considered pathogens of veterinary medical interest. In this chapter, we present general diagnostic workflows for sampling livestock animals to assess STEC prevalence, magnitude, and duration of host colonization. This is followed by detailed method protocols for STEC detection and typing at genetic and phenotypic levels to assess the relative virulence exerted by the strains.

Indexed as

Cattle DiseasesEscherichia coli InfectionsShiga-Toxigenic Escherichia coliSwine DiseasesAnimalsCattleShiga Toxin 2SwineShiga Toxin 2Adhesion assayELISAHost–cell interactionsInvasion assayMultilocus sequence typingPCRPrevalencePulsed-field gel electrophoresisShiga toxinShiga toxin-producing Escherichia coliSTECVero cell cytotoxicity assayVirulence genes

Identifiers

PMID33704748

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.