Evidence map›Paper›PMID 33704727›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Fate Mapping of Cancer Cells in Metastatic Lymph Nodes Using Photoconvertible Proteins.

Ethel R Pereira, Dmitriy Kedrin, Timothy P Padera

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ethel R PereiraEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital (MGH) Research Institute, MGH and Harvard Medical School (HMS), Boston, MA, USA.
Dmitriy KedrinEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital (MGH) Research Institute, MGH and Harvard Medical School (HMS), Boston, MA, USA.
Timothy P PaderaEdwin L. Steele Laboratories, Department of Radiation Oncology, Massachusetts General Hospital (MGH) Research Institute, MGH and Harvard Medical School (HMS), Boston, MA, USA. tpadera@steele.mgh.harvard.edu.

Funding

Systems biology of lymphatic transportR01HL128168 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI MUNN, LANCE L., PADERA, TIMOTHY P · 2015 to 2019
$2.9M
Characterizing lymphatic micrometastases: prognostic and therapeutic implicationsDP2OD008780 · OD · MASSACHUSETTS GENERAL HOSPITAL · PI PADERA, TIMOTHY P · 2011 to 2011
$2.6M
Targeting lymph node metastases to prevent cancer progressionR01CA214913 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI PADERA, TIMOTHY P · 2017 to 2022
$2.0M
NCI NIH HHS R01 CA214913NHLBI NIH HHS R01 HL128168NIH HHS DP2 OD008780
6 · The paper itself

Abstract

The lymph node microenvironment is extremely dynamic and responds to immune stimuli in the host by reprogramming immune, stromal, and endothelial cells. In normal physiological conditions, the lymph node will initiate an appropriate immune response to clear external threats that the host may experience. However, in metastatic disease, cancer cells often colonize local lymph nodes, disrupt immune function, and even leave the lymph node to create additional metastases. Understanding how cancer cells enter, colonize, survive, proliferate, and interact with other cell types in the lymph node is challenging. Here, we describe the use of photoconvertible fluorescent proteins to label and trace the fate of cancer cells once they enter the lymph node.

Indexed as

Cell TrackingLymph NodesNeoplasms, ExperimentalNeoplastic Cells, CirculatingOptical ImagingAnimalsCell Line, TumorGreen Fluorescent ProteinsHumansLymphatic MetastasisMiceTumor MicroenvironmentGreen Fluorescent ProteinsCirculating tumor cellsConfocal microscopyDendra2Intravital imagingLymph nodeMetastasisPhotoconvertible proteinsPhotodiode

Identifiers

PMID33704727
PMCPMC8924909

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.