Evidence map›Paper›PMID 33704720›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Isolation and Quantification of Plasma Circulating Tumor DNA from Melanoma Patients.

Gabriela Marsavela, Anna Reid, Elin S Gray, Leslie Calapre

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Gabriela MarsavelaSchool of Medical and Health Sciences, Edith Cowan University, Joondalup, WA, Australia.
Anna ReidSchool of Medical and Health Sciences, Edith Cowan University, Joondalup, WA, Australia.
Elin S GraySchool of Medical and Health Sciences, Edith Cowan University, Joondalup, WA, Australia. e.gray@ecu.edu.au.
Leslie CalapreSchool of Medical and Health Sciences, Edith Cowan University, Joondalup, WA, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In recent years, circulating tumor DNA (ctDNA) has emerged as a promising prognostic and monitoring biomarker of various cancers, including melanoma. However, sensitive methods are required for its preservation, isolation, and detection. Here we describe a sensitive method for plasma ctDNA isolation using a column-based extraction kit, followed by quantification using a single mutational target with a droplet digital PCR system. This sensitive protocol has been successfully used to quantify diverse mutations present in plasma-derived ctDNA from cancer patients. The full procedure, from blood processing to the analysis of results, takes approximately a day of work.

Indexed as

Biomarkers, TumorCirculating Tumor DNADNA, NeoplasmHumansMelanomaPlasmaPolymerase Chain ReactionProto-Oncogene Proteins B-rafBiomarkers, TumorCirculating Tumor DNADNA, NeoplasmProto-Oncogene Proteins B-rafCell-free DNA (cfDNA)Circulating tumor DNA (ctDNA )Droplet digital PCR (ddPCR)

Identifiers

PMID33704720

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.