Evidence map›Paper›PMID 33704586›Full record

ArticleJournal of plant research2021

Targeted mutagenesis of two homologous ATP-binding cassette subfamily G (ABCG) genes in tomato confers resistance to parasitic weed Phelipanche aegyptiaca.

Vinay Kumar Bari, Jackline Abu Nassar, Ayala Meir, Radi Aly

Abstract read
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In one paragraph

Article in Journal of plant research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.7field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. Article
  3. Transcriptomic Approach for Investigation ofCurrent issues in molecular biology · 2024
    Article
  4. Article
  5. Review
  6. Review
  7. Characterization of a Chickpea Mutant Resistant toPlants (Basel, Switzerland) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Vinay Kumar BariDepartment of Plant Pathology and Weed Research, Newe Ya'ar Research Center, Agricultural Research Organization (ARO), Ramat Yishay, Israel. vinay.bari@cup.edu.in.ORCID http://orcid.org/0000-0002-5670-3798
Jackline Abu NassarDepartment of Plant Pathology and Weed Research, Newe Ya'ar Research Center, Agricultural Research Organization (ARO), Ramat Yishay, Israel.
Ayala MeirDepartment of Plant Pathology and Weed Research, Newe Ya'ar Research Center, Agricultural Research Organization (ARO), Ramat Yishay, Israel.
Radi AlyDepartment of Plant Pathology and Weed Research, Newe Ya'ar Research Center, Agricultural Research Organization (ARO), Ramat Yishay, Israel. radi@volcani.agri.gov.il.ORCID http://orcid.org/0000-0002-6038-9586
Agricultural Research Organization · ILCentral University of Punjab · IN

Funding

ARO Volcani Center 132-1919-18
6 · The paper itself

Abstract

Phelipanche aegyptiaca and Orobanche spp. are obligate plant root-parasitic weeds that cause extensive damage in agricultural crop plants. Their germination requires exposure to strigolactones (SLs) exuded by the host plant roots. Here we studied genes in the host plant tomato involved in SL exudation and their impact on parasitic weeds. We provide evidence that CRISPR/Cas9-mediated targeted mutagenesis of two homologous ATP-binding cassette subfamily G (ABCG) genes, ABCG44 (Solyc08g067610) and ABCG45 (Solyc08g067620), in tomato significantly reduces SLs in the root exudate and abolishes germination of the root-parasitic weed P. aegyptiaca. Based on genome sequence similarity between ABCG44 and ABCG45, a 20-bp target sequence in their exon region was selected to design single guide RNA targeting both genes using CRISPR/Cas9. The plant binary vector constructs harboring the specific Cas9 and single guide RNA were transformed into tomato. Selected T

Indexed as

OrobancheSolanum lycopersicumAdenosine TriphosphateGerminationMutagenesisPlant RootsPlant WeedsAdenosine TriphosphateATP-binding cassette (ABC) transporterCRISPR/Cas9 genome editingOrobancholParasitic plantStrigolactone

Identifiers

PMID33704586
OpenAlexW3134839457

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.