ArticleeLife2021
tTARGIT AAVs mediate the sensitive and flexible manipulation of intersectional neuronal populations in mice.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- Beyond satiety: unraveling the complex roles of POMC neurons in behavior and metabolism.Reviews in endocrine & metabolic disorders · 2026Review
- Increased leptin signaling drives the response of hypothalamic LepRb neurons to diet-induced obesity.Molecular metabolism · 2026Article
- Molecularly defined subpopulations of leptin receptor neurons dissociate the control of food intake from blood pressure.bioRxiv : the preprint server for biology · 2026Article
- A genetically-defined population of amygdalofugal neurons promotes suckling and early postnatal growth.bioRxiv : the preprint server for biology · 2025Article
- Neuropeptides as transmitters and regulators of the locus coeruleus noradrenergic system.Pharmacological reviews · 2025Review
- Control of physiologic glucose homeostasis via hypothalamic modulation of gluconeogenic substrate availability.Molecular metabolism · 2025Article
- Molecular mechanisms and neural mediators of leptin action.Genes & development · 2025Review
- Article
- A single-vector intersectional AAV strategy for interrogating cellular diversity and brain function.Nature neuroscience · 2024Article
- Ventromedial hypothalamic nucleus subset stimulates tissue thermogenesis via preoptic area outputs.Molecular metabolism · 2024Article
- A Non-canonical Excitatory PV RGC-PV SC Visual Pathway for Mediating the Looming-evoked Innate Defensive Response.Neuroscience bulletin · 2024Article
- Article
- A clock-dependent brake for rhythmic arousal in the dorsomedial hypothalamus.Nature communications · 2023Article
- Suppression of food intake by Glp1r/Lepr-coexpressing neurons prevents obesity in mouse models.The Journal of clinical investigation · 2023Article
- Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle.Scientific reports · 2023Article
- AAV- based vector improvements unrelated to capsid protein modification.Frontiers in medicine · 2023Review
- The ventromedial hypothalamic nucleus: watchdog of whole-body glucose homeostasis.Cell & bioscience · 2022Review
- Cre driver mouse lines for thalamocortical circuit mapping.The Journal of comparative neurology · 2022Article
- Strategies for Targeting Neural Circuits: How to Manipulate Neurons Using Virus Vehicles.Frontiers in neural circuits · 2022Review
- ExBoX - a simple Boolean exclusion strategy to drive expression in neurons.Journal of cell science · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
While Cre-dependent viral systems permit the manipulation of many neuron types, some cell populations cannot be targeted by a single DNA recombinase. Although the combined use of Flp and Cre recombinases can overcome this limitation, insufficient recombinase activity can reduce the efficacy of existing Cre+Flp-dependent viral systems. We developed a sensitive dual recombinase-activated viral approach: tTA-driven Recombinase-Guided Intersectional Targeting (tTARGIT) adeno-associated viruses (AAVs). tTARGIT AAVs utilize a Flp-dependent tetracycline transactivator (tTA) 'Driver' AAV and a tetracycline response element-driven, Cre-dependent 'Payload' AAV to express the transgene of interest. We employed this system in
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.