Evidence map›Paper›PMID 33704064›Full record

ArticleeLife2021

Reformulation of an extant ATPase active site to mimic ancestral GTPase activity reveals a nucleotide base requirement for function.

Taylor B Updegrove, Jailynn Harke, Vivek Anantharaman, Jin Yang, Nikhil Gopalan, Di Wu, Grzegorz Piszczek, David M Stevenson, Daniel Amador-Noguez, Jue D Wang and 2 more

Open access · goldAbstract read
In one paragraph

Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. A lipid cue drives the subcellular localization of a self-inserting bacterial transmembrane protein.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
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  7. Bacterial spore surface nanoenvironment requires a AAA+ ATPase to promote MurG function.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  8. Article
  9. Article
  10. Article
  11. Cell division machinery drives cell-specific gene activation during differentiation inProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  12. Enhanced TROSY Effect in [2-Angewandte Chemie (International ed. in English) · 2024
    Article
  13. Article
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  15. Article
  16. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Taylor B Updegrove *Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, United States.
Jailynn Harke *Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, United States.
Vivek AnantharamanNational Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, United States.ORCID 0000-0001-8395-0009
Jin YangDepartment of Bacteriology, University of Wisconsin, Madison, United States.
Nikhil GopalanLaboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, United States.
Di WuBiophysics Core Facility, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, United States.
Grzegorz PiszczekBiophysics Core Facility, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, United States.
David M StevensonDepartment of Bacteriology, University of Wisconsin, Madison, United States.
Daniel Amador-NoguezDepartment of Bacteriology, University of Wisconsin, Madison, United States.
Jue D WangDepartment of Bacteriology, University of Wisconsin, Madison, United States.ORCID 0000-0003-1503-170X
L AravindNational Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, United States.ORCID 0000-0003-0771-253X
Kumaran S RamamurthiLaboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, United States.ORCID 0000-0002-2335-3568
National Institutes of Health · USUniversity of Wisconsin–Madison · US

Funding

Subcellular protein localization in B. subtilisZIABC011211 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI RAMAMURTHI, KUMARAN · 2009 to 2025
$21.4M
Evolutionary Analysis and Comparative Genomics of Protein SuperfamiliesZIALM594244 · NLM · NATIONAL LIBRARY OF MEDICINE · PI ARAVIND, L · 2009 to 2025
$20.3M
The critical roles of (p)ppGpp in homeostasis and antibiotic tolerance in Gram positive bacteriaR35GM127088 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Jue D. Wang · 2018 to 2026
$3.9M
NIGMS NIH HHS R35 GM127088
6 · The paper itself

Abstract

Hydrolysis of nucleoside triphosphates releases similar amounts of energy. However, ATP hydrolysis is typically used for energy-intensive reactions, whereas GTP hydrolysis typically functions as a switch. SpoIVA is a bacterial cytoskeletal protein that hydrolyzes ATP to polymerize irreversibly during

Indexed as

Adenosine TriphosphatasesAdenosine TriphosphateBacillus subtilisBacterial ProteinsCatalytic DomainGTP PhosphohydrolasesGuanosine TriphosphateHydrolysisPolymerizationProtein EngineeringAdenosine TriphosphatasesAdenosine TriphosphateBacterial ProteinsGTP PhosphohydrolasesGuanosine Triphosphatespore-specific proteins, BacillusactinB. subtiliscell biologyinfectious diseasemicrobiologyMreBppGppseptinsSpoVMtubulin

Identifiers

PMID33704064
PMCPMC7952092
OpenAlexW3134291098

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.