ArticleNucleic acids research2021
Interrogating accessibility of telomeric sequences with FRET-PAINT: evidence for length-dependent telomere compaction.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 16 citations in OpenAlex.
- Accessibility of telomeric overhangs to stabilizing small-molecule ligands.Nucleic acids research · 2026Article
- Accessibility of Telomeric Overhangs to Stabilizing Small-Molecule Ligands.bioRxiv : the preprint server for biology · 2025Article
- Impact of Molecular Crowding on Accessibility of Telomeric Overhangs Forming Multiple G-Quadruplexes.Biomacromolecules · 2025Article
- Impact of Molecular Crowding on Accessibility of Telomeric Overhangs Forming Multiple G-quadruplexes.bioRxiv : the preprint server for biology · 2025Article
- Core-Extended Naphthalene Diimide Dyads as Light-Up Probes with Targeted Cytotoxicity Toward Tumor Cells.Biomolecules · 2025Article
- Protection of the Telomeric Junction by the Shelterin Complex.Journal of the American Chemical Society · 2024Article
- Protection of the Telomeric Junction by the Shelterin Complex.bioRxiv : the preprint server for biology · 2024Article
- Structure, Topology, and Stability of Multiple G-quadruplexes in Long Telomeric Overhangs.Journal of molecular biology · 2024Review
- Detecting secondary structure formation with FRET-PAINT.The Analyst · 2023Article
- Inhibited complete folding of consecutive human telomeric G-quadruplexes.Nucleic acids research · 2023Article
- Shelterin reduces the accessibility of telomeric overhangs.Nucleic acids research · 2022Article
- In Situ Super-Resolution Imaging of Telomeres with DNA-PAINT.ACS omega · 2022Article
- Emerging accessibility patterns in long telomeric overhangs.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Editorial: Single-molecule studies of DNA-protein interactions collection 2021.Nucleic acids research · 2021Article
- Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules.Micromachines · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Single-stranded telomeric overhangs are ∼200 nucleotides long and can form tandem G-quadruplex (GQ) structures, which reduce their accessibility to nucleases and proteins that activate DNA damage response. Whether these tandem GQs further stack to form compact superstructures, which may provide better protection for longer telomeres, is not known. We report single-molecule measurements where the accessibility of 24-144 nucleotide long human telomeric DNA molecules is interrogated by a short PNA molecule that is complementary to a single GGGTTA repeat, as implemented in the FRET-PAINT method. Binding of the PNA strand to available GGGTTA sequences results in discrete FRET bursts which were analyzed in terms of their dwell times, binding frequencies, and topographic distributions. The binding frequencies were greater for binding to intermediate regions of telomeric DNA compared to 3'- or 5'-ends, suggesting these regions are more accessible. Significantly, the binding frequency per telomeric repeat monotonically decreased with increasing telomere length. These results are consistent with telomeres forming more compact structures at longer lengths, reducing accessibility of these critical genomic sites.
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