ArticleNucleic acids research2021
Long-read sequencing and de novo genome assemblies reveal complex chromosome end structures caused by telomere dysfunction at the single nucleotide level.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- ONT-only genome assembly of a Korean male individual using a semen sample.Genes & genomics · 2026Article
- Quantifying telomere length: from bulk assays to single-molecule resolution.Biophysics reports · 2026Article
- Resolving repetitive and telomeric regions through a high-contiguity genome assembly of the African Turquoise killifish.Genes & genomics · 2026Article
- Micronuclei: origins, assays, mechanisms, diseases and treatments.Signal transduction and targeted therapy · 2026Review
- Adaptation of centromeres to breakage through local genomic and epigenomic remodeling in wheat.Genome research · 2025Article
- Assembly of a high-quality Polypterus senegalus diploid genome.Genes & genomics · 2025Article
- Genome assembly resources of genitourinary cancers for chromosomal aberration at the single nucleotide level.Scientific data · 2025Article
- GDBr: genomic signature interpretation tool for DNA double-strand break repair mechanisms.Nucleic acids research · 2025Article
- Highly accurate Korean draft genomes reveal structural variation highlighting human telomere evolution.Nucleic acids research · 2025Article
- Units containing telomeric repeats are prevalent in subtelomeric regions of a Mesorhabditis isolate collected from the Republic of Korea.Genes & genomics · 2024Article
- Haplotype-resolved de novo assembly revealed unique characteristics of alternative lengthening of telomeres in mouse embryonic stem cells.Nucleic acids research · 2024Article
- Telomerase-independent survival leads to a mosaic of complex subtelomere rearrangements inGenome research · 2023Article
- Experimental systems for the analysis of mutational signatures: no 'one-size-fits-all' solution.Biochemical Society transactions · 2023Article
- Benchmarking datasets for assembly-based variant calling using high-fidelity long reads.BMC genomics · 2023Article
- A beginner's guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies.STAR protocols · 2022Article
- Celebrating Mendel, McClintock, and Darlington: On end-to-end chromosome fusions and nested chromosome fusions.The Plant cell · 2022Review
- IntraspecificNAR genomics and bioinformatics · 2022Article
- Long-read sequencing infers a mechanism for copy number variation of template for alternative lengthening of telomeres in a wildmicroPublication biology · 2022Article
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4 authors.
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Abstract
Karyotype change and subsequent evolution is triggered by chromosome fusion and rearrangement events, which often occur when telomeres become dysfunctional. Telomeres protect linear chromosome ends from DNA damage responses (DDRs), and telomere dysfunction may result in genome instability. However, the complex chromosome end structures and the other possible consequences of telomere dysfunction have rarely been resolved at the nucleotide level due to the lack of the high-throughput methods needed to analyse these highly repetitive regions. Here we applied long-read sequencing technology to Caenorhabditis elegans survivor lines that emerged after telomere dysfunction. The survivors have preserved traces of DDRs in their genomes and our data revealed that variants generated by telomere dysfunction are accumulated along all chromosomes. The reconstruction of the chromosome end structures through de novo genome assemblies revealed diverse types of telomere damage processing at the nucleotide level. When telomeric repeats were totally eroded by telomere dysfunction, DDRs were mostly terminated by chromosome fusion events. We also partially reconstructed the most complex end structure and its DDR signatures, which would have been accumulated via multiple cell divisions. These finely resolved chromosome end structures suggest possible mechanisms regarding the repair processes after telomere dysfunction, providing insights into chromosome evolution in nature.
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