ArticleJournal of industrial microbiology & biotechnology2021
Enhanced production of clavulanic acid by improving glycerol utilization using reporter-guided mutagenesis of an industrial Streptomyces clavuligerus strain.
Article in Journal of industrial microbiology & biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Enhancing microbial metabolic capacity through high-energy electron beam-induced intense structural variations.Nature communications · 2026Article
- Integrated genomic-transcriptomic analysis of clavulanic acid production in differentially productive Streptomyces clavuligerus strains.Scientific reports · 2025Article
- Genomic Diversity ofInternational journal of molecular sciences · 2024Article
- Sequence and origin of the Streptomyces intergenetic-conjugation helper plasmid pUZ8002.Access microbiology · 2024Article
- Transcriptome profiles of Streptomyces clavuligerus strains producing different titers of clavulanic acid.Scientific data · 2023Article
- DnaK-Mediated Protein Deamidation: a Potential Mechanism for Virulence and Stress Adaptation in Cronobacter sakazakii.Applied and environmental microbiology · 2023Article
- Improvement of rimocidin production in Streptomyces rimosus M527 by reporter-guided mutation selection.Journal of industrial microbiology & biotechnology · 2023Article
- Molecular mechanism of GylR-mediated regulation of glycerol metabolism inFrontiers in microbiology · 2022Article
- Streptomyces clavuligerus: The Omics Era.Journal of industrial microbiology & biotechnology · 2021Article
- Introduction to the special issue: "Natural Product Discovery and Development in the Genomic Era: 2021".Journal of industrial microbiology & biotechnology · 2021Article
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6 authors.
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Abstract
Clavulanic acid (CA) produced by Streptomyces clavuligerus is a clinically important β-lactamase inhibitor. It is known that glycerol utilization can significantly improve cell growth and CA production of S. clavuligerus. We found that the industrial CA-producing S. clavuligerus strain OR generated by random mutagenesis consumes less glycerol than the wild-type strain; we then developed a mutant strain in which the glycerol utilization operon is overexpressed, as compared to the parent OR strain, through iterative random mutagenesis and reporter-guided selection. The CA production of the resulting S. clavuligerus ORUN strain was increased by approximately 31.3% (5.21 ± 0.26 g/l) in a flask culture and 17.4% (6.11 ± 0.36 g/l) in a fermenter culture, as compared to that of the starting OR strain. These results confirmed the important role of glycerol utilization in CA production and demonstrated that reporter-guided mutant selection is an efficient method for further improvement of randomly mutagenized industrial strains.
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