Observational studyThe Journal of nutrition2021
Personalized Profiling Reveals Donor- and Lactation-Specific Trends in the Human Milk Proteome and Peptidome.
Observational study in The Journal of nutrition, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- A Proteomic Atlas of Human Milk: Uncovering Milk Functional Components.Research square · 2026Article
- Central role of glycosylation processes in human genetic susceptibility to SARS-CoV-2 infections with Omicron variants.Nature genetics · 2026Article
- Dynamic Variations in Endogenous Peptides in Chinese Human Milk Across Lactation and Geographical Regions.Nutrients · 2025Article
- Industrial Scale Production and Characterization of a Whey Fraction Enriched in Extracellular Vesicle Material.Journal of extracellular biology · 2025Article
- Robust and High-Resolution All-Ion Fragmentation LC-ESI-IM-MS Analysis for In-Depth Characterization or Profiling of Up to 200 Human Milk Oligosaccharides.Analytical chemistry · 2025Article
- The ferroform and glycoform landscape of human milk lactoferrin dissected through hybrid mass spectrometric approaches.Frontiers in nutrition · 2025Article
- Optimizing workflow efficiency for analyzing low molecular weight endogenous peptides in colostrum.RSC advances · 2024Article
- Influence of Early Life Factors, including breast milk Composition, on the Microbiome of Infants Born to Mothers with and without Inflammatory Bowel Disease.Journal of Crohn's & colitis · 2023Article
- Fingerprinting of Proteases, Protease Inhibitors and Indigenous Peptides in Human Milk.Nutrients · 2023Article
- Identification of common and distinct origins of human serum and breastmilk IgA1 by mass spectrometry-based clonal profiling.Cellular & molecular immunology · 2023Article
- The Effect of In Utero Exposure to Maternal Inflammatory Bowel Disease and Immunomodulators on Infant Immune System Development and Function.Cellular and molecular gastroenterology and hepatology · 2023Review
- Cellular and transcriptional diversity over the course of human lactation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Exploring Human Milk Dynamics: Interindividual Variation in Milk Proteome, Peptidome, and Metabolome.Journal of proteome research · 2022Article
- Changes in Antisecretory Factor in Human Milk During the Postpartum and Length of Gestation.Journal of human lactation : official journal of International Lactation Consultant Association · 2022Article
- Maternal vaccination as an additional approach to improve the protection of the nursling: Anti-infective properties of breast milk.Clinics (Sao Paulo, Brazil) · 2022Review
- Proteoform Profiles Reveal That Alpha-1-Antitrypsin in Human Serum and Milk Is Derived From a Common Source.Frontiers in molecular biosciences · 2022Article
- Human milk nutritional composition across lactational stages in Central Africa.Frontiers in nutrition · 2022Article
- Longitudinal changes of lactopontin (milk osteopontin) in term and preterm human milk.Frontiers in nutrition · 2022Article
- Analysis of the Endogenous Peptidomes of Different Infant Formula Types and Human Milk.Foods (Basel, Switzerland) · 2021Article
- Optimization of a human milk-directed quantitative sIgA ELISA method substantiated by mass spectrometry.Analytical and bioanalytical chemistry · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman milk is the most genuine form of personalized nutrition, whereby its nutritional and bioactive constituents support the changing needs of the growing infant. Personalized proteome profiling strategies may provide insights into maternal-infant relationships. Proteins and endogenous peptides in human milk play an important role as nutrients for growth and have distinct functionality such as immune defense. Comprehensive monitoring of all of the human milk proteinaceous components, including endogenous peptides, is required to fully understand the changing role of the human milk proteome throughout lactation.
objectiveWe aimed to investigate the personalized nature of the human milk proteome and peptidome for individual mother-infant dyads.
methodsTwo individual healthy milk donors, aged 29 and 32 y and both of a normal BMI, were longitudinally observed over weeks 1, 2, 3, 4, 6, 8, 10, 12, and 16 postpartum. Milk collection was standardized. Comprehensive variations in the human milk proteinaceous components were assessed using quantitative LC-MS/MS methods.
resultsWe longitudinally profiled the concentrations of >1300 milk proteins and 2000 endogenous milk peptides spanning 16 wk of lactation for 2 individual donors. We observed many gradual and alike changes in both donors related to temporal effects, for instance early lactation was marked by high concentrations of proteins and peptides involved in lactose synthesis and immune development. Uniquely, in 1 of the 2 donors, we observed a substantial anomaly in the milk composition, exclusively at week 6, likely indicating a response to inflammation and/or infection.
conclusionsHere, we provide a resource for characterizing the lactational changes in the human milk proteome, encompassing thousands of proteins and endogenous peptides. Further, we demonstrate the feasibility and benefit of personalized profiling to monitor the influence of milk on the development of the newborn, as well as the health status of each individual mother-infant pair.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.