Evidence map›Paper›PMID 33693300›Full record

ArticleTranslational medicine of aging2018

A toolkit for DNA assembly, genome engineering and multicolor imaging for

Bryan Sands, Nikolay Burnaevskiy, Soo R Yun, Mathew M Crane, Matt Kaeberlein, Alexander Mendenhall

Open access · goldAbstract read
In one paragraph

Article in Translational medicine of aging, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.3field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 20 citations in OpenAlex.

  1. bioRxiv : the preprint server for biology · 2026
    Article
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  5. Observing and Quantifying Fluorescent Reporters.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  6. Article
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  8. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Bryan SandsDepartment of Pathology, University of Washington, Seattle, Washington, United States of America.
Nikolay BurnaevskiyDepartment of Pathology, University of Washington, Seattle, Washington, United States of America.
Soo R YunDepartment of Pathology, University of Washington, Seattle, Washington, United States of America.
Mathew M CraneDepartment of Pathology, University of Washington, Seattle, Washington, United States of America.
Matt KaeberleinDepartment of Pathology, University of Washington, Seattle, Washington, United States of America.
Alexander MendenhallDepartment of Pathology, University of Washington, Seattle, Washington, United States of America.
University of Washington · US

Funding

THERAPEUTIC EFFECTS OF INTRA-NASAL INSULIN DETEMIRP50AG005136 · NIA · UNIVERSITY OF WASHINGTON · PI GRABOWSKI, THOMAS J. · 1985 to 2019
$57.2M
SPECIFICITY OF OXYGEN DNA DAMAGE AND MUTAGENESISP01AG001751 · NIA · UNIVERSITY OF WASHINGTON · PI PRUSKY, GLEN THOMAS · 1985 to 2021
$29.6M
University of Washington Nathan Shock Center of Excellence in the Basic Biology of AgingP30AG013280 · NIA · UNIVERSITY OF WASHINGTON · PI Maitreya J Dunham · 1995 to 2026
$27.1M
GENETIC APPROACHES TO AGING RESEARCHT32AG000057 · NIA · UNIVERSITY OF WASHINGTON · PI RABINOVITCH, PETER S · 1985 to 2019
$14.2M
Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Ann E. Rougvie · 2012 to 2026
$7.5M
Causes and consequences of lifespan biomarker variation in Caenorhabditis elegansR00AG045341 · NIA · UNIVERSITY OF WASHINGTON · PI MENDENHALL, ALEXANDER RICHARD · 2016 to 2018
$747k
NIA NIH HHS P01 AG001751NIA NIH HHS P30 AG013280NIA NIH HHS P50 AG005136NIA NIH HHS R00 AG045341NIA NIH HHS T32 AG000057NIH HHS P40 OD010440
6 · The paper itself

Abstract

One way scientists can observe and quantify processes in living cells is to engineer the genomes of animals to express multiple fluorescent proteins and then quantify those signals by various imaging techniques. To allow our laboratories to confidently quantify mixed (overlapping) fluorescent signals for our studies in the basic biology of gene expression and aging in

Identifiers

PMID33693300
PMCPMC7943185
OpenAlexW2802260035

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.