ArticlePLoS genetics2021
Changes in both trans- and cis-regulatory elements mediate insecticide resistance in a lepidopteron pest, Spodoptera exigua.
Article in PLoS genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 85 citations in OpenAlex.
- Functional analysis of down-regulated CYP6AE gene clusters involved in the insecticidal mechanism of lycorine against Spodoptera litura.Pest management science · 2026Article
- Cuticle thickening mediates insecticide penetration resistance in Spodoptera litura.Journal of advanced research · 2025Article
- Article
- Mechanisms and Genetic Drivers of Resistance of Insect Pests to Insecticides and Approaches to Its Control.Toxics · 2025Review
- Expression reduction and a variant of a P450 gene mediate chlorpyrifos resistance in Tetranychus urticae Koch.Journal of advanced research · 2025Article
- Cuticle protein mediates the evolution of stress resistance by generating a decoy circular RNA in spider mite.Science advances · 2025Article
- Two independent regulatory mechanisms synergistically contribute to P450-mediated insecticide resistance in a lepidopteran pest, Spodoptera exigua.BMC biology · 2025Article
- CRISPR/Cas Technology in Insect Insecticide Resistance.Insects · 2025Review
- Inhibition of transcriptional regulation of detoxification genes contributes to insecticide resistance management in Spodoptera exigua.Communications biology · 2025Article
- The ecdysone-induced bZIP transcription factor MafB establishes a positive feedback loop to enhance vitellogenesis and reproduction in theProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Self-assembled co-delivery nanoplatform for increasing the broad-spectrum susceptibility of fall armyworm toward insecticides.Journal of advanced research · 2025Article
- Unveiling the Role of Two Rhodopsin-like GPCR Genes in Insecticide-Resistant House Flies,International journal of molecular sciences · 2024Article
- Parallel Evolution in Mosquito Vectors-A Duplicated Esterase Locus is Associated With Resistance to Pirimiphos-methyl in Anopheles gambiae.Molecular biology and evolution · 2024Article
- Keap1 Negatively Regulates Transcription of Three Counter-Defense Genes and Susceptibility to Plant Toxin Gossypol inInsects · 2024Article
- Transcriptional regulation and overexpression of GST cluster enhances pesticide resistance in the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae).Communications biology · 2023Article
- A nuclear receptor HR96-related gene underlies large trans-driven differences in detoxification gene expression in a generalist herbivore.Nature communications · 2023Article
- The pleiotropic AMPK-CncC signaling pathway regulates the trade-off between detoxification and reproduction.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Multiple-P450 Gene Co-Up-Regulation in the Development of Permethrin Resistance in the House Fly,International journal of molecular sciences · 2023Article
- Transcriptome profile of halofuginone resistant and sensitive strains ofFrontiers in microbiology · 2023Article
- Enthralling genetic regulatory mechanisms meddling insecticide resistance development in insects: role of transcriptional and post-transcriptional events.Frontiers in molecular biosciences · 2023Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The evolution of insect resistance to insecticides is frequently associated with overexpression of one or more cytochrome P450 enzyme genes. Although overexpression of CYP450 genes is a well-known mechanism of insecticide resistance, the underlying regulatory mechanisms are poorly understood. Here we uncovered the mechanisms of overexpression of the P450 gene, CYP321A8 in a major pest insect, Spodoptera exigua that is resistant to multiple insecticides. CYP321A8 confers resistance to organophosphate (chlorpyrifos) and pyrethroid (cypermethrin and deltamethrin) insecticides in this insect. Constitutive upregulation of transcription factors CncC/Maf are partially responsible for upregulated expression of CYP321A8 in the resistant strain. Reporter gene assays and site-directed mutagenesis analyses demonstrated that CncC/Maf enhanced the expression of CYP321A8 by binding to specific sites in the promoter. Additional cis-regulatory elements resulting from a mutation in the CYP321A8 promoter in the resistant strain facilitates the binding of the orphan nuclear receptor, Knirps, and enhances the promoter activity. These results demonstrate that two independent mechanisms; overexpression of transcription factors and mutations in the promoter region resulting in a new cis-regulatory element that facilitates binding of the orphan nuclear receptor are involved in overexpression of CYP321A8 in insecticide-resistant S. exigua.
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