Evidence map›Paper›PMID 33689166›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Assessment of Blood-Brain Barrier Permeability Using Miniaturized Fluorescence Microscopy in Freely Moving Rats.

Jeffrey L Barr, G Cristina Brailoiu, Ellen M Unterwald, Eugen Brailoiu

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Modulation of the Blood-Brain Barrier by Sigma-1R Activation.International journal of molecular sciences · 2024
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jeffrey L BarrCenter for Substance Abuse Research, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, USA.
G Cristina BrailoiuDepartment of Pharmaceutical Sciences, Jefferson College of Pharmacy, Thomas Jefferson University, Philadelphia, PA, USA.
Ellen M UnterwaldCenter for Substance Abuse Research, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, USA. ellen.unterwald@temple.edu.
Eugen BrailoiuCenter for Substance Abuse Research, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, USA. ebrailou@temple.edu.

Funding

Pilot Projects Core (PPC)P30DA013429 · NIDA · TEMPLE UNIV OF THE COMMONWEALTH · PI Munir Gunes Kutlu · 2000 to 2026
$34.6M
NIDA NIH HHS P30 DA013429
6 · The paper itself

Abstract

We report here the method of visualization of brain microcirculation and assessment of blood-brain barrier (BBB) permeability changes using the miniature integrated fluorescence microscope (i.e., miniscope) technology in awake, freely moving rats. The imaging cannula is implanted in the brain area of interest of anesthetized adult rats. After recovery and habituation, sodium fluorescein, a low-molecular-weight tracer, is injected i.v. Fluorescence intensity in the vicinity of microvessels, as an indicator of BBB permeability, is then recorded in vivo via the miniscope for extended periods of time. The method can be used to assess the changes in BBB permeability produced by pharmacologic agents; in this case, the drug of interest is administered after sodium fluorescein. An increase in the sodium fluorescein extravasation in brain microcirculation demonstrates an increase in BBB permeability. The method described here allows a high-resolution visualization of real-time changes in BBB permeability in awake, freely moving rats.

Indexed as

Blood-Brain BarrierBrainAnimalsCapillary PermeabilityFluoresceinMicroscopy, FluorescencePermeabilityRatsFluoresceinBrain microcirculationFluorescence imagingIn vivo BBB permeabilitySodium fluoresceinVascular extravasation

Identifiers

PMID33689166

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.