ArticleProceedings of the National Academy of Sciences of the United States of America2021
Synthetic heparan sulfate standards and machine learning facilitate the development of solid-state nanopore analysis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Enzymatically hydrolyzed oligosaccharide fingerprinting using nanopipettes at the single-molecule level.Chemical science · 2026Article
- A universal deep learning framework for empowering nanopore identification by reinforcing temporal signals.Nature communications · 2026Article
- Extracellular matrix remodelling in neurological diseases.Nature reviews. Neurology · 2026Review
- Glycan Sequencing, A Brief Primer.Glycoscience & therapy · 2026Article
- Nanopore profiling and structure determination of oligosaccharides by fragmentation.Science advances · 2026Article
- Dermatan Sulfate: Structure, Biosynthesis, and Biological Roles.Biomolecules · 2025Review
- Amplification-Free Quantification of Endogenous Mitochondrial DNA Copy Number Using Solid-State Nanopores.ACS nano · 2025Article
- Designing nanotheranostics with machine learning.Nature nanotechnology · 2024Review
- Characterizing Prion-Like Protein Aggregation: Emerging Nanopore-Based Approaches.Small methods · 2024Review
- A Robust Parallel Computing Data Extraction Framework for Nanopore Experiments.Small methods · 2024Article
- Research Progress on Saccharide Molecule Detection Based on Nanopores.Sensors (Basel, Switzerland) · 2024Review
- Editors' Choice-Perspective-Deciphering the Glycan Kryptos by Solid-State Nanopore Single-Molecule Sensing: A Call for Integrated Advancements Across Glyco- and Nanopore Science.ECS sensors plus · 2024Article
- Nanopore-based glycan sequencing: state of the art and future prospects.Chemical science · 2024Review
- Probing nanopore surface chemistry through real-time measurements of nanopore conductance response to pH changes.The Review of scientific instruments · 2023Article
- Analysis of Nanopore Data: Classification Strategies for an Unbiased Curation of Single-Molecule Events from DNA Nanostructures.ACS sensors · 2023Article
- Identification of tagged glycans with a protein nanopore.Nature communications · 2023Article
- Glycosaminoglycans: What Remains To Be Deciphered?JACS Au · 2023Review
- Portable nanopore-sequencing technology: Trends in development and applications.Frontiers in microbiology · 2023Review
- AutoNanopore: An Automated Adaptive and Robust Method to Locate Translocation Events in Solid-State Nanopore Current Traces.ACS omega · 2022Article
- Comprehensive structural assignment of glycosaminoglycan oligo- and polysaccharides by protein nanopore.Nature communications · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The application of solid-state (SS) nanopore devices to single-molecule nucleic acid sequencing has been challenging. Thus, the early successes in applying SS nanopore devices to the more difficult class of biopolymer, glycosaminoglycans (GAGs), have been surprising, motivating us to examine the potential use of an SS nanopore to analyze synthetic heparan sulfate GAG chains of controlled composition and sequence prepared through a promising, recently developed chemoenzymatic route. A minimal representation of the nanopore data, using only signal magnitude and duration, revealed, by eye and image recognition algorithms, clear differences between the signals generated by four synthetic GAGs. By subsequent machine learning, it was possible to determine disaccharide and even monosaccharide composition of these four synthetic GAGs using as few as 500 events, corresponding to a zeptomole of sample. These data suggest that ultrasensitive GAG analysis may be possible using SS nanopore detection and well-characterized molecular training sets.
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