Evidence map›Paper›PMID 33688018›Full record

ArticlemSystems2021

Direct Nanopore Sequencing of mRNA Reveals Landscape of Transcript Isoforms in Apicomplexan Parasites.

V Vern Lee, Louise M Judd, Aaron R Jex, Kathryn E Holt, Christopher J Tonkin, Stuart A Ralph

Open access · goldAbstract read
In one paragraph

Article in mSystems, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 48 citations in OpenAlex.

  1. Review
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  8. Characterization of co-infections of haemosporidian parasites in SwinhoeCurrent research in parasitology & vector-borne diseases · 2025
    Article
  9. Proteomic identification of aFEMS microbes · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

V Vern LeeDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Victoria, Australia.
Louise M JuddDepartment of Infectious Diseases, Central Clinical School, Monash University, Melbourne, Victoria, Australia.
Aaron R JexThe Walter and Eliza Hall Institute of Medical Research, Parkville, Melbourne, Victoria, Australia.ORCID https://orcid.org/0000-0002-1285-7947
Kathryn E HoltDepartment of Infectious Diseases, Central Clinical School, Monash University, Melbourne, Victoria, Australia.ORCID https://orcid.org/0000-0003-3949-2471
Christopher J TonkinThe Walter and Eliza Hall Institute of Medical Research, Parkville, Melbourne, Victoria, Australia.ORCID https://orcid.org/0000-0002-7036-6222
Stuart A RalphDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Victoria, Australia saralph@unimelb.edu.au.ORCID https://orcid.org/0000-0003-0114-7808
The University of Melbourne · AULondon School of Hygiene & Tropical Medicine · GBMonash University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alternative splicing is a widespread phenomenon in metazoans by which single genes are able to produce multiple isoforms of the gene product. However, this has been poorly characterized in apicomplexans, a major phylum of some of the most important global parasites. Efforts have been hampered by atypical transcriptomic features, such as the high AU content of

Indexed as

nanoporePlasmodiumRNA-seqRNA splicingToxoplasmatranscriptional regulation

Identifiers

PMID33688018
PMCPMC8561664
OpenAlexW3133817684

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.