ArticleAngewandte Chemie (International ed. in English)2021
Less Unfavorable Salt Bridges on the Enzyme Surface Result in More Organic Cosolvent Resistance.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Computer-Aided Lipase Engineering for Improving Their Stability and Activity in the Food Industry: State of the Art.Molecules (Basel, Switzerland) · 2023Pooled it
- Investigation of salt-bridge interactions underlying salt activation of halophilic methionine sulfoxide reductase A.Frontiers in microbiology · 2026Article
- Effect of acyl donors on EGCG esterification reaction catalyzed by Lipase "Amano" 30SD based on molecular dynamics analysis.Current research in food science · 2026Article
- Efficient 2,3-Butanediol Production from Ethanol by a Modified Four-Enzyme Synthetic Biosystem.Molecules (Basel, Switzerland) · 2024Article
- Solvent concentration at 50% protein unfolding may reform enzyme stability ranking and process window identification.Nature communications · 2024Article
- Solvent Tolerance Improvement of Lipases Enhanced Their Applications: State of the Art.Molecules (Basel, Switzerland) · 2024Review
- Engineering diaryl alcohol dehydrogenase KpADH reveals importance of retaining hydration shell in organic solvent tolerance.Protein science : a publication of the Protein Society · 2024Article
- A Hydrolase Produced byToxins · 2023Article
- Enhanced Activity and Stability of an Acetyl Xylan Esterase in Hydrophilic Alcohols through Site-Directed Mutagenesis.Molecules (Basel, Switzerland) · 2023Article
- Discovery and mechanism-guided engineering of BHET hydrolases for improved PET recycling and upcycling.Nature communications · 2023Article
- Employing non-canonical amino acids towards the immobilization of a hyperthermophilic enzyme to increase protein stability.RSC advances · 2023Article
- Rational design of enzyme activity and enantioselectivity.Frontiers in bioengineering and biotechnology · 2023Review
- A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions.Angewandte Chemie (International ed. in English) · 2022Article
- Polar Substitutions on the Surface of a Lipase Substantially Improve Tolerance in Organic Solvents.ChemSusChem · 2022Article
- Immobilization of Eversa Lipases on Hydrophobic Supports for Ethanolysis of Sunflower Oil Solvent-Free.Applied biochemistry and biotechnology · 2022Article
- Article
- Critical assessment of structure-based approaches to improve protein resistance in aqueous ionic liquids by enzyme-wide saturation mutagenesis.Computational and structural biotechnology journal · 2022Article
- Less Unfavorable Salt Bridges on the Enzyme Surface Result in More Organic Cosolvent Resistance.Angewandte Chemie (International ed. in English) · 2021Article
- Aqueous ionic liquids redistribute local enzyme stability via long-range perturbation pathways.Computational and structural biotechnology journal · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biocatalysis for the synthesis of fine chemicals is highly attractive but usually requires organic (co-)solvents (OSs). However, native enzymes often have low activity and resistance in OSs and at elevated temperatures. Herein, we report a smart salt bridge design strategy for simultaneously improving OS resistance and thermostability of the model enzyme, Bacillus subtilits Lipase A (BSLA). We combined comprehensive experimental studies of 3450 BSLA variants and molecular dynamics simulations of 36 systems. Iterative recombination of four beneficial substitutions yielded superior resistant variants with up to 7.6-fold (D64K/D144K) improved resistance toward three OSs while exhibiting significant thermostability (thermal resistance up to 137-fold, and half-life up to 3.3-fold). Molecular dynamics simulations revealed that locally refined flexibility and strengthened hydration jointly govern the highly increased resistance in OSs and at 50-100 °C. The salt bridge redesign provides protein engineers with a powerful and likely general approach to design OSs- and/or thermal-resistant lipases and other α/β-hydrolases.
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