A 3D Dynamic In Vitro Model of Inflammatory Tendon Disease.

Susanna Schubert, Luisa Brandt, Janina Burk

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Susanna SchubertInstitute of Human Genetics, University of Leipzig Medical Center, Leipzig, Germany. Susanna.schubert@medizin.uni-leipzig.de.

Luisa BrandtSaxon Incubator for Clinical Translation (SIKT), University of Leipzig, Leipzig, Germany.

Janina BurkSaxon Incubator for Clinical Translation (SIKT), University of Leipzig, Leipzig, Germany. Janina.Burk@vetmed.uni-giessen.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Three-dimensional (3D) cell cultures combining multipotent mesenchymal stromal cells (MSC), tendon extracellular matrix scaffolds, and mechanical stimulation by a bioreactor have been used to induce tenogenic differentiation in vitro. Yet, these conditions alone do not mimic the environment of acute inflammatory tendon disease adequately, thus the results of such studies are not representatives for tendon regeneration after acute injury. In this chapter, we describe two different approaches to introduce inflammatory stimuli, comprising co-culture with leukocytes and supplementation with the cytokines IL-1 β and TNF-α. The presented in vitro model of inflammatory tendon disease could be used to study musculoskeletal pathophysiology and regeneration in more depth.

Indexed as

Models, BiologicalAnimalsHumansInflammationInterleukin-1betaMesenchymal Stem CellsTendinopathyTendonsTissue ScaffoldsTumor Necrosis Factor-alphaIL1B protein, humanInterleukin-1betaTumor Necrosis Factor-alphaHorseInterleukin-1 (IL-1)LeukocytesMesenchymal stromal cellsRegenerative medicineScaffoldTenogenic DifferentiationTissue engineeringTumor necrosis factor-α (TNF-α)

Identifiers

PMID33687679

DOI10.1016/j.tvjl.2012.06.004

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.