Evidence map›Paper›PMID 33687572›Full record

ArticleMikrochimica acta2021

Nuclease-assisted target recycling signal amplification strategy for graphene quantum dot-based fluorescent detection of marine biotoxins.

Huajie Gu, Liling Hao, Hua Ye, Pengfei Ma, Zhouping Wang

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Article in Mikrochimica acta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.5field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Huajie GuSchool of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou, 215009, People's Republic of China.
Liling HaoSchool of Medical Instrument and Food Engineering, Shanghai Engineering Research Center for Food Rapid Detection, University of Shanghai for Science and Technology, Shanghai, 200093, People's Republic of China.
Hua YeState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Pengfei MaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Zhouping WangState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China. wangzp@jiangnan.edu.cn.
State Key Laboratory of Food Science and Technology · CNJiangnan University · CNUniversity of Shanghai for Science and Technology · CN

Funding

Jiangsu Agricultural Science and Technology Innovation Fund CX (18)2025National Natural Science Foundation of China 31871881Natural Science Foundation of Shanghai 18ZR1409500Science and Technology Support Program of Jiangsu Province BE2017623the National First-class Discipline Program of Food Science and Technology JUFSTR20180303the Technology Research and Development Program of Suzhou SNG2018044
6 · The paper itself

Abstract

Saxitoxin (STX) is a major marine toxin from shellfish, and it is responsible for paralytic shellfish poisoning (PSP). In this study, a highly sensitive and rapid aptamer assay was developed for STX detection by combining fluorescence resonance energy transfer (FRET) and nuclease-assisted target recycling signal amplification. The aptamer STX-41 conjugated with graphene quantum dots (GQDs) was adsorbed on magnetic reduced graphene oxide (MRGO) to establish a fluorescence quenching system. Then, the binding between STX and aptamer induced the desorption of GQD-aptamer from MRGO and the restoring of fluorescence for the fluorescent determination of STX. The digestion of the target bound aptamer by DNase I could release the target for recycling thus achieving signal amplification. Under the optimized conditions, the aptamer assay showed a wide detection range (0.1-100 ng·mL

Indexed as

AnimalsAptamers, NucleotideBiosensing TechniquesBivalviaEndodeoxyribonucleasesFluorescence Resonance Energy TransferFluorescent DyesFood ContaminationGraphiteLimit of DetectionMagnetic PhenomenaQuantum DotsSaxitoxinAptamers, NucleotideEndodeoxyribonucleasesFluorescent Dyesgraphene oxideGraphiteSaxitoxinAptamerDNase I-catalyzed target recycling signal amplificationFluorescence resonance energy transferGraphene quantum dotSaxitoxin

Identifiers

PMID33687572
OpenAlexW3134725927

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.