ArticleAnalytical chemistry2021
Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging.
Article in Analytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- Spatial lipidomics of the human brain: systematic review of current state and future perspectives.Molecular psychiatry · 2026Pooled it
- Improvements in high-yield detection and localisation of cholesterol in murine brains using water cluster beam secondary ion mass spectrometry.Analytical and bioanalytical chemistry · 2026Article
- Aziridination-Enabled Structural and Quantitative Lipidomics.Journal of the American Society for Mass Spectrometry · 2026Review
- Cholesterol nose-to-brain delivery as a possible therapeutic strategy in Huntington's disease.Translational neurodegeneration · 2026Article
- Sterol biosynthesis, brain development, and disease.The Journal of clinical investigation · 2026Review
- New Analytical Technologies to Resolve, Interpret, and Understand Lipid Complexity.Annual review of analytical chemistry (Palo Alto, Calif.) · 2026Review
- MALDI Matrix: Origins, Innovations, and Frontiers.Chemical reviews · 2026Review
- Spatially Resolved Lipid Composition of the Human Brain Cortical Layers.Neuroscience bulletin · 2026Article
- Enhancing Hydroxyl Metabolite Analysis through In Situ Derivatization-Enabled Desorption Electrospray Ionization-Mass Spectrometry.Analytical chemistry · 2025Article
- Topological segmentation of mass spectrometry imaging data.Journal of mass spectrometry and advances in the clinical lab · 2025Article
- Innovative Application of a Multifunctional Sucrose-Gelatin Hydrogel Matrix in Desorption Electrospray Ionization-Mass Spectrometry Imaging.Analytical chemistry · 2025Article
- Sterol imbalances and cholesterol-24-hydroxylase dysregulation is linked to the underlying progression of multiple sclerosis.Brain pathology (Zurich, Switzerland) · 2025Article
- High-Specificity Spatiotemporal Cholesterol Detection by Quadrature Phase-Shifted Polarization Stimulated Raman Imaging.Angewandte Chemie (International ed. in English) · 2025Article
- Advances in mass spectrometry of lipids for the investigation of Niemann-pick type C disease.Lipids in health and disease · 2025Review
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- The importance of choosing the appropriate cholesterol quantification method: enzymatic assay versus gas chromatography.Journal of lipid research · 2024Article
- Quantitative mass spectrometry imaging (qMSI): A tutorial.Journal of mass spectrometry : JMS · 2024Article
- Spatial neurolipidomics-MALDI mass spectrometry imaging of lipids in brain pathologies.Journal of mass spectrometry : JMS · 2024Review
- Quantitative mass spectrometry imaging: therapeutics & biomolecules.Chemical communications (Cambridge, England) · 2024Review
- Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?Biomolecules · 2024Review
Corrections and comments
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Authors and funding
19 authors at 6 institutions in 3 countries.
Funding
Abstract
Despite being a critical molecule in the brain, mass spectrometry imaging (MSI) of cholesterol has been under-reported compared to other lipids due to the difficulty in ionizing the sterol molecule. In the present work, we have employed an on-tissue enzyme-assisted derivatization strategy to improve detection of cholesterol in brain tissue sections. We report distribution and levels of cholesterol across specific structures of the mouse brain, in a model of Niemann-Pick type C1 disease, and during brain development. MSI revealed that in the adult mouse, cholesterol is the highest in the pons and medulla and how its distribution changes during development. Cholesterol was significantly reduced in the corpus callosum and other brain regions in the
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.