ArticleThe New phytologist2021
Direct phosphorylation of HY5 by SPA kinases to regulate photomorphogenesis in Arabidopsis.
Article in The New phytologist, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 58 citations in OpenAlex.
- Phosphorylation of the plant photoreceptor UVR8 regulates its dimer/monomer status and enhances UV-B signaling across species.The Plant cell · 2026Article
- Antagonistic regulation of PIF4 and HY5 activity by BBX28 regulates thermomorphogenesis.Science advances · 2026Article
- Conservation and divergence of UVR8 photoreceptor-mediated UV-B signaling in Marchantia polymorpha.Plant physiology · 2026Article
- Twenty-five years of photobodies: formation, composition, and the 2-compartment logic of phytochrome B signaling.Plant physiology · 2026Review
- Involvement of the Light Signalling Components HY5 and BIC1,2 and SPA1 in Plant Responses to Elevated Daytime UV-B Doses.International journal of molecular sciences · 2026Article
- Article
- Genome-wide analysis of theFrontiers in plant science · 2026Article
- Beyond light signaling: the broad regulatory spectrum of AFPs in plant growth and development.Frontiers in plant science · 2026Review
- A PIF-regulated switch in cell axis growth drives cotyledon expansion through tissue-specific cell expansion and division.The Plant journal : for cell and molecular biology · 2025Article
- TmCOP1-TmHY5 module-mediated blue light signal promotes chicoric acid biosynthesis in Taraxacum mongolicum.Plant biotechnology journal · 2025Article
- Shedding light on iron nutrition: exploring intersections of transcription factor cascades in light and iron deficiency signaling.Journal of experimental botany · 2025Review
- The PEAPOD repressor complex in Arabidopsis stomatal development.Frontiers in plant science · 2025Review
- Spatiotemporal bifurcation of HY5-mediated blue-light signaling regulates wood development during secondary growth.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Ubiquitin-specific protease UBP14 stabilizes HY5 by deubiquitination to promote photomorphogenesis inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The phosphorylation of carboxyl-terminal eIF2α by SPA kinases contributes to enhanced translation efficiency during photomorphogenesis.Nature communications · 2024Article
- COP1 controls light-dependent chromatin remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- reduced internode 1 shortens internode length while increasing soybean yield.Plant communications · 2024Article
- Phosphorylation of Arabidopsis UVR8 photoreceptor modulates protein interactions and responses to UV-B radiation.Nature communications · 2024Article
- Root-specific photoreception directs early root development by HY5-regulated ROS balance.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Characterization of PIF4 Phosphorylation by SPA1.Methods in molecular biology (Clifton, N.J.) · 2024Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Elongated hypocotyl5 (HY5) is a key transcription factor that promotes photomorphogenesis. Constitutive photomorphogenic1 (COP1)-Suppressor of phytochrome A-105 (SPA) E3 ubiquitin ligase complex promotes ubiquitination and degradation of HY5 to repress photomorphogenesis in darkness. HY5 is also regulated by phosphorylation at serine 36 residue. However, the kinase responsible for phosphorylation of HY5 remains unknown. Here, using extensive in vitro and in vivo biochemical, genetic, and photobiological techniques, we have identified a new kinase that phosphorylates HY5 and demonstrated the significance of phosphorylation of HY5 in Arabidopsis thaliana. We show that SPA proteins are the missing kinases necessary for HY5 phosphorylation. SPAs can directly phosphorylate HY5 in vitro, and the phosphorylated HY5 is absent in the spaQ background in vivo. We also demonstrate that the unphosphorylated HY5 interacts strongly with both COP1 and SPA1 and is the preferred substrate for degradation, whereas the phosphorylated HY5 is more stable in the dark. In addition, the unphosphorylated HY5 actively binds to the target promoters and is the physiologically more active form. Consistently, the transgenic plants expressing the unphosphorylated form of HY5 display enhanced photomorphogenesis. Collectively, our study revealed the missing kinase responsible for direct phosphorylation of HY5 that fine-tunes its stability and activity to regulate photomorphogenesis.
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