Evidence map›Paper›PMID 33686254›Full record

ArticleGene therapy2021

Mesenchymal stem cells overexpressing BMP-9 by CRISPR-Cas9 present high in vitro osteogenic potential and enhance in vivo bone formation.

Gileade P Freitas, Helena B Lopes, Alann T P Souza, Maria Paula O Gomes, Georgia K Quiles, Jonathan Gordon, Coralee Tye, Janet L Stein, Gary S Stein, Jane B Lian and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gene therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 44 citations in OpenAlex.

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  19. Bone Regeneration and Repair Materials.Journal of functional biomaterials · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Gileade P Freitas *Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.ORCID http://orcid.org/0000-0002-1674-4667
Helena B Lopes *Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Alann T P SouzaBone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Maria Paula O GomesBone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.ORCID http://orcid.org/0000-0002-4466-0794
Georgia K QuilesBone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Jonathan GordonDepartment of Biochemistry, University of Vermont School of Medicine, Burlington, VT, USA.
Coralee TyeDepartment of Biochemistry, University of Vermont School of Medicine, Burlington, VT, USA.ORCID http://orcid.org/0000-0002-3349-1833
Janet L SteinDepartment of Biochemistry, University of Vermont School of Medicine, Burlington, VT, USA.ORCID http://orcid.org/0000-0002-2474-6822
Gary S SteinDepartment of Biochemistry, University of Vermont School of Medicine, Burlington, VT, USA.
Jane B LianDepartment of Biochemistry, University of Vermont School of Medicine, Burlington, VT, USA.
Marcio M BelotiBone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Adalberto L RosaBone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil. adalrosa@forp.usp.br.ORCID http://orcid.org/0000-0002-6495-2778
Universidade de São Paulo · BRUniversity of Vermont · USUniversidade de Ribeirão Preto · BR

Funding

Understanding Factors Influencing COVID-19 Testing and Vaccination in Immigrant Low-income and Homeless Populations and Testing Targeted InterventionsU54GM115516 · NIGMS · MAINEHEALTH · PI CLIFFORD JAMES ROSEN · 2017 to 2026
$51.6M
CONTROL OF OSTEOBLAST PROLIFERATION AND DIFFERENTIATIONR01AR039588 · NIAMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI LIAN, JANE B., STEIN, GARY S. · 1990 to 2019
$8.0M
Chromatin Organization Regulates OsteogenesisR01DE029311 · NIDCR · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI LIAN, JANE B., STEIN, GARY S. · 2020 to 2024
$2.5M
NIAMS NIH HHS R01 AR039588NIDCR NIH HHS R01 DE029311NIGMS NIH HHS U54 GM115516
6 · The paper itself

Abstract

Cell therapy is a valuable strategy for the replacement of bone grafts and repair bone defects, and mesenchymal stem cells (MSCs) are the most frequently used cells. This study was designed to genetically edit MSCs to overexpress bone morphogenetic protein 9 (BMP-9) using Clustered Regularly Interspaced Short Palindromic Repeats/associated nuclease Cas9 (CRISPR-Cas9) technique to generate iMSCs-VPR

Indexed as

Growth Differentiation Factor 2Mesenchymal Stem CellsOsteogenesisAnimalsCell DifferentiationCells, CulturedCRISPR-Cas SystemsRatsGrowth Differentiation Factor 2

Identifiers

PMID33686254
PMCPMC8423866
OpenAlexW3134333094

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.