ArticleCommunications biology2021
A flat embedding method for transmission electron microscopy reveals an unknown mechanism of tetracycline.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Probing Antibiotic Inhibition in Small Bacterial Populations With Combinatorial Droplet Microfluidics.Small science · 2026Article
- Desiccation tolerance in peatland desmids: a comparative study of Micrasterias thomasiana and Staurastrum hirsutum (Zygnematophyceae).Protoplasma · 2025Article
- TamL is a Key Player of the Outer Membrane Homeostasis in Bacteroidota.Journal of molecular biology · 2025Article
- LolA and LolB are conserved in Bacteroidota and are crucial for gliding motility and Type IX secretion.Communications biology · 2025Article
- Second harmonic scattering investigation of bacterial efflux induced by the antibiotic tetracycline.The Journal of chemical physics · 2024Article
- Potent and specific antibiotic combination therapy against Clostridioides difficile.Nature chemical biology · 2024Article
- Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction.Nature communications · 2024Article
- Antimicrobial Activity of 2-(Piperazin-1-yl)naphtho[2,3-d]thiazole-4,9-dione against Staphylococcus Strains.Molecules (Basel, Switzerland) · 2024Article
- Facilitating flip-flop: Structural tuning of molecule-membrane interactions in living bacteria.Biophysical journal · 2023Article
- Antimicrobial Activity of an Fmoc-Plantaricin 149 Derivative Peptide against Multidrug-Resistant Bacteria.Antibiotics (Basel, Switzerland) · 2023Article
- Dual Action of Eeyarestatin 24 on Sec-Dependent Protein Secretion and Bacterial DNA.ACS infectious diseases · 2023Article
- Tracking Global and Local Changes in Membrane Fluidity Through Fluorescence Spectroscopy and Microscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Monitoring membranes: The exploration of biological bilayers with second harmonic generation.Chemical physics reviews · 2022Review
- Mechanistic Studies andJournal of medicinal chemistry · 2022Article
- Effect of g-CEcotoxicology (London, England) · 2022Article
- Mechanistic insights into the CChemical science · 2022Article
- Article
- Isolation of Persister Cells ofInternational journal of molecular sciences · 2021Article
- Control of septum thickness by the curvature of SepF polymers.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Multitarget Approaches against Multiresistant Superbugs.ACS infectious diseases · 2020Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transmission electron microscopy of cell sample sections is a popular technique in microbiology. Currently, ultrathin sectioning is done on resin-embedded cell pellets, which consumes milli- to deciliters of culture and results in sections of randomly orientated cells. This is problematic for rod-shaped bacteria and often precludes large-scale quantification of morphological phenotypes due to the lack of sufficient numbers of longitudinally cut cells. Here we report a flat embedding method that enables observation of thousands of longitudinally cut cells per single section and only requires microliter culture volumes. We successfully applied this technique to Bacillus subtilis, Escherichia coli, Mycobacterium bovis, and Acholeplasma laidlawii. To assess the potential of the technique to quantify morphological phenotypes, we monitored antibiotic-induced changes in B. subtilis cells. Surprisingly, we found that the ribosome inhibitor tetracycline causes membrane deformations. Further investigations showed that tetracycline disturbs membrane organization and localization of the peripheral membrane proteins MinD, MinC, and MreB. These observations are not the result of ribosome inhibition but constitute a secondary antibacterial activity of tetracycline that so far has defied discovery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.