Evidence map›Paper›PMID 33686099›Full record

ArticleScientific reports2021

Coculture with hiPS-derived intestinal cells enhanced human hepatocyte functions in a pneumatic-pressure-driven two-organ microphysiological system.

Marie Shinohara, Hiroshi Arakawa, Yuuichi Oda, Nobuaki Shiraki, Shinji Sugiura, Takumi Nishiuchi, Taku Satoh, Keita Iino, Sylvia Leo, Yusuke Kato and 15 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 31 citations in OpenAlex.

  1. Review
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  4. Novel emerging cell and organoid systems for the study of drug metabolism and toxicity in humans.Drug metabolism and disposition: the biological fate of chemicals · 2025
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  14. Microtechnology-basedAPL bioengineering · 2021
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors at 5 institutions in 1 country.

Marie ShinoharaInstitute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Hiroshi ArakawaFaculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
Yuuichi OdaInstitute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Nobuaki ShirakiSchool of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan.
Shinji SugiuraBiotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Takumi NishiuchiAdvanced Science Research Centre, Kanazawa University, Kanazawa, Japan.
Taku SatohBiotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Keita IinoSchool of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan.
Sylvia LeoSchool of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan.
Yusuke KatoSchool of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan.
Karin ArayaFaculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
Takumi KawanishiFaculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
Tomoki NakatsujiFaculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
Manami MitsutaDepartment of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Kosuke InamuraDepartment of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Tomomi GotoDepartment of Mechanical Engineering, School of Engineering, Tokai University, Kanagawa, Japan.
Kenta ShinhaDepartment of Mechanical Engineering, School of Engineering, Tokai University, Kanagawa, Japan.
Wataru NiheiDepartment of Mechanical Engineering, School of Engineering, Tokai University, Kanagawa, Japan.
Kikuo KomoriDepartment of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Masaki NishikawaDepartment of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Shoen KumeSchool of Life Science and Technology, Tokyo Institute of Technology, Kanagawa, Japan.
Yukio KatoFaculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
Toshiyuki KanamoriBiotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Yasuyuki SakaiDepartment of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Hiroshi KimuraInstitute of Industrial Science, The University of Tokyo, Tokyo, Japan. hkimura@tokai-u.jp.
The University of Tokyo · JPKanazawa University · JPTokyo Institute of Technology · JPTokai University · JPNational Institute of Advanced Industrial Science and Technology · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Examining intestine-liver interactions is important for achieving the desired physiological drug absorption and metabolism response in in vitro drug tests. Multi-organ microphysiological systems (MPSs) constitute promising tools for evaluating inter-organ interactions in vitro. For coculture on MPSs, normal cells are challenging to use because they require complex maintenance and careful handling. Herein, we demonstrated the potential of coculturing normal cells on MPSs in the evaluation of intestine-liver interactions. To this end, we cocultured human-induced pluripotent stem cell-derived intestinal cells and fresh human hepatocytes which were isolated from PXB mice with medium circulation in a pneumatic-pressure-driven MPS with pipette-friendly liquid-handling options. The cytochrome activity, albumin production, and liver-specific gene expressions in human hepatocytes freshly isolated from a PXB mouse were significantly upregulated via coculture with hiPS-intestinal cells. Our normal cell coculture shows the effects of the interactions between the intestine and liver that may occur in vivo. This study is the first to demonstrate the coculturing of hiPS-intestinal cells and fresh human hepatocytes on an MPS for examining pure inter-organ interactions. Normal-cell coculture using the multi-organ MPS could be pursued to explore unknown physiological mechanisms of inter-organ interactions in vitro and investigate the physiological response of new drugs.

Indexed as

Lab-On-A-Chip DevicesMicrofluidic Analytical TechniquesAnimalsCoculture TechniquesDrug Evaluation, PreclinicalHepatocytesHumansInduced Pluripotent Stem CellsMicePressure

Identifiers

PMID33686099
PMCPMC7940409
OpenAlexW3135131086

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.