Evidence map›Paper›PMID 33685924›Full record

ArticleDiabetes2021

Single Molecule-Based fliFISH Validates Radial and Heterogeneous Gene Expression Patterns in Pancreatic Islet β-Cells.

Fangjia Li, Dehong Hu, Cailin Dieter, Charles Ansong, Lori Sussel, Galya Orr

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diabetes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Impact of anFrontiers in endocrinology · 2023
    Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Fangjia LiEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA.
Dehong HuEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA.
Cailin DieterBarbara Davis Center for Diabetes, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO.
Charles AnsongBiological Sciences Division, Pacific Northwest National Laboratory, Richland, WA.
Lori SusselBarbara Davis Center for Diabetes, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO galya.orr@pnnl.gov lori.sussel@cuanschutz.edu.
Galya OrrEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA galya.orr@pnnl.gov lori.sussel@cuanschutz.edu.ORCID 0000-0002-5552-2151
Pacific Northwest National Laboratory · USUniversity of Colorado Anschutz Medical Campus · US

Funding

Alternative RNA splicing events contribute to the onset of islet dysfunction in T1DU01DK127505 · NIDDK · UNIVERSITY OF COLORADO DENVER · PI NAKAYASU, ERNESTO SATOSHI, SUSSEL, LORI · 2020 to 2023
$2.9M
Single Cell Resolution Omics Analysis of T1D isletsUC4DK108101 · NIDDK · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI ANSONG, CHARLES K, SUSSEL, LORI · 2015 to 2015
$2.9M
NIDDK NIH HHS U01 DK127505NIDDK NIH HHS UC4 DK108101
6 · The paper itself

Abstract

Single-cell RNA-sequencing (scRNA-Seq) technologies have greatly enhanced our understanding of islet cell transcriptomes and have revealed the existence of β-cell heterogeneity. However, comparison of scRNA-Seq data sets from different groups have highlighted inconsistencies in gene expression patterns, primarily due to variable detection of lower abundance transcripts. Furthermore, such analyses are unable to uncover the spatial organization of heterogeneous gene expression. In this study, we used fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) to quantify transcripts in single cells in mouse pancreatic islet sections. We compared the expression patterns of Insulin 2 (

Indexed as

AnimalsIn Situ Hybridization, FluorescenceInsulin-Secreting CellsMaf Transcription Factors, LargeMiceRGS ProteinsRNA-SeqSequence Analysis, RNASingle-Cell AnalysisUrocortinsMafa protein, mouseMaf Transcription Factors, LargeRGS4 proteinRGS ProteinsUcn3 protein, mouseUrocortins

Identifiers

PMID33685924
PMCPMC8173798
OpenAlexW3135516100

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.