ArticleBMC microbiology2021
Tolerance of Yarrowia lipolytica to inhibitors commonly found in lignocellulosic hydrolysates.
Article in BMC microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 63 citations in OpenAlex.
- Coupled intracellular redox and extracellular respiration sensing for quantitative oxidative stress profiling.bioRxiv : the preprint server for biology · 2026Article
- Isolation of Candida maltosa strains able to achieve a high lipid productivity from malt bagasse hemicellulosic hydrolysate.World journal of microbiology & biotechnology · 2026Article
- The oleaginous yeast Cutaneotrichosporon oleaginosum modifies corn stover alkali lignin.Scientific reports · 2026Article
- Engineering de novo formatotrophy in the non-model yeastNature synthesis · 2026Article
- An Evolved Strain of the Oleaginous YeastJournal of fungi (Basel, Switzerland) · 2023Article
- Cinnamic acid and p-coumaric acid are metabolized to 4-hydroxybenzoic acid by Yarrowia lipolytica.AMB Express · 2023Article
- Xylose consumption and ethanol production by Pichia guilliermondii and Candida oleophila in the presence of furans, phenolic compounds, and organic acids commonly produced during the pre-treatment of plant biomass.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Yarrowia lipolytica produces lipid-rich biomass in medium mimicking lignocellulosic biomass hydrolysate.Applied microbiology and biotechnology · 2023Article
- Evolving tolerance of Yarrowia lipolytica to hydrothermal liquefaction aqueous phase waste.Applied microbiology and biotechnology · 2023Article
- Abolishing storage lipids induces protein misfolding and stress responses in Yarrowia lipolytica.Journal of industrial microbiology & biotechnology · 2023Article
- Advances in the Application of the Non-Conventional YeastJournal of fungi (Basel, Switzerland) · 2023Review
- Article
- Advanced one-pot deconstruction and valorization of lignocellulosic biomass into triacetic acid lactone using Rhodosporidium toruloides.Microbial cell factories · 2022Article
- Validated Growth Rate-Dependent Regulation of Lipid Metabolism inInternational journal of molecular sciences · 2022Article
- Exploring Yeast Diversity to Produce Lipid-Based Biofuels from Agro-Forestry and Industrial Organic Residues.Journal of fungi (Basel, Switzerland) · 2022Review
- Improving Lipid Production ofInternational journal of molecular sciences · 2022Article
- Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels.Bioengineered · 2022Review
- Considering Strain Variation and Non-Type Strains for Yeast Metabolic Engineering Applications.Life (Basel, Switzerland) · 2022Review
- Formate Dehydrogenase Improves the Resistance to Formic Acid and Acetic Acid Simultaneously inInternational journal of molecular sciences · 2022Article
- Strategies to increase tolerance and robustness of industrial microorganisms.Synthetic and systems biotechnology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLignocellulosic material is a suitable renewable carbon and energy source for microbial cell factories, such as Yarrowia lipolytica. To be accessible for microorganisms, the constituent sugars need to be released in a hydrolysis step, which as a side effect leads to the formation of various inhibitory compounds. However, the effects of these inhibitory compounds on the growth of Y. lipolytica have not been thoroughly investigated.
resultsHere we show the individual and combined effect of six inhibitors from three major inhibitor groups on the growth of Y. lipolytica. We engineered a xylose consuming strain by overexpressing the three native genes XR, XDH, and XK and found that the inhibitor tolerance of Y. lipolytica is similar in glucose and in xylose. Aromatic compounds could be tolerated at high concentrations, while furfural linearly increased the lag phase of the cultivation, and hydroxymethylfurfural only inhibited growth partially. The furfural induced increase in lag phase can be overcome by an increased volume of inoculum. Formic acid only affected growth at concentrations above 25 mM. In a synthetic hydrolysate, formic acid, furfural, and coniferyl aldehyde were identified as the major growth inhibitors.
conclusionWe showed the individual and combined effect of inhibitors found in hydrolysate on the growth of Y. lipolytica. Our study improves understanding of the growth limiting inhibitors found in hydrolysate and enables a more targeted engineering approach to increase the inhibitor tolerance of Y. lipolytica. This will help to improve the usage of Y. lipolytica as a sustainable microbial cell factory.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.